Isolation of spermidine synthase gene (spsA) of Dictyostelium discoideum

Kunde Guo, Wen-Tsan Chang, Peter C. Newell

Research output: Contribution to journalArticle

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Abstract

The gene encoding spermidine synthase (spsA) was isolated from Dictyostelium discoideum using the technique of insertional mutagenesis. Northern blot analysis showed that the spsA mRNA is expressed maximally during the vegetative stage and decreases gradually during the 24 h of development. Sequencing of the genomic DNA and a full-length cDNA clone indicated the presence of one intron in a gene coding for a predicted protein (SpsA) with 284 amino acids. The sequence is highly conserved, with amino acid identities compared to spermidine synthases of humans, 59.5%, to mouse, 61.3%, and to yeast, 58.1%. A null mutant of the spsA gene is unable to grow in the absence of exogenous spermidine. Development of spsA null cells grown in the absence of spermidine produced fruiting bodies that have abnormally short stalks. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1449
Issue number3
DOIs
Publication statusPublished - 1999 Apr 1

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Spermidine Synthase
Dictyostelium
Genes
Spermidine
Amino Acids
Null Lymphocytes
Insertional Mutagenesis
DNA Sequence Analysis
Northern Blotting
Introns
Complementary DNA
Clone Cells
Yeasts
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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Isolation of spermidine synthase gene (spsA) of Dictyostelium discoideum. / Guo, Kunde; Chang, Wen-Tsan; Newell, Peter C.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1449, No. 3, 01.04.1999, p. 211-216.

Research output: Contribution to journalArticle

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