TY - JOUR
T1 - Isolation of spermidine synthase gene (spsA) of Dictyostelium discoideum
AU - Guo, Kunde
AU - Chang, Wen Tsan
AU - Newell, Peter C.
N1 - Funding Information:
We thank Kei Inouye, Julian D. Gross and Gunilla A. Lundberg for helpful advice, and the BBSRC for financial support.
PY - 1999/4/1
Y1 - 1999/4/1
N2 - The gene encoding spermidine synthase (spsA) was isolated from Dictyostelium discoideum using the technique of insertional mutagenesis. Northern blot analysis showed that the spsA mRNA is expressed maximally during the vegetative stage and decreases gradually during the 24 h of development. Sequencing of the genomic DNA and a full-length cDNA clone indicated the presence of one intron in a gene coding for a predicted protein (SpsA) with 284 amino acids. The sequence is highly conserved, with amino acid identities compared to spermidine synthases of humans, 59.5%, to mouse, 61.3%, and to yeast, 58.1%. A null mutant of the spsA gene is unable to grow in the absence of exogenous spermidine. Development of spsA null cells grown in the absence of spermidine produced fruiting bodies that have abnormally short stalks. Copyright (C) 1999 Elsevier Science B.V.
AB - The gene encoding spermidine synthase (spsA) was isolated from Dictyostelium discoideum using the technique of insertional mutagenesis. Northern blot analysis showed that the spsA mRNA is expressed maximally during the vegetative stage and decreases gradually during the 24 h of development. Sequencing of the genomic DNA and a full-length cDNA clone indicated the presence of one intron in a gene coding for a predicted protein (SpsA) with 284 amino acids. The sequence is highly conserved, with amino acid identities compared to spermidine synthases of humans, 59.5%, to mouse, 61.3%, and to yeast, 58.1%. A null mutant of the spsA gene is unable to grow in the absence of exogenous spermidine. Development of spsA null cells grown in the absence of spermidine produced fruiting bodies that have abnormally short stalks. Copyright (C) 1999 Elsevier Science B.V.
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U2 - 10.1016/S0167-4889(99)00018-X
DO - 10.1016/S0167-4889(99)00018-X
M3 - Article
C2 - 10209300
AN - SCOPUS:0032960912
SN - 0167-4889
VL - 1449
SP - 211
EP - 216
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 3
ER -