Isolation of spermidine synthase gene (spsA) of Dictyostelium discoideum

Kunde Guo, Wen Tsan Chang, Peter C. Newell

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


The gene encoding spermidine synthase (spsA) was isolated from Dictyostelium discoideum using the technique of insertional mutagenesis. Northern blot analysis showed that the spsA mRNA is expressed maximally during the vegetative stage and decreases gradually during the 24 h of development. Sequencing of the genomic DNA and a full-length cDNA clone indicated the presence of one intron in a gene coding for a predicted protein (SpsA) with 284 amino acids. The sequence is highly conserved, with amino acid identities compared to spermidine synthases of humans, 59.5%, to mouse, 61.3%, and to yeast, 58.1%. A null mutant of the spsA gene is unable to grow in the absence of exogenous spermidine. Development of spsA null cells grown in the absence of spermidine produced fruiting bodies that have abnormally short stalks. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)211-216
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Issue number3
Publication statusPublished - 1999 Apr 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Isolation of spermidine synthase gene (spsA) of Dictyostelium discoideum'. Together they form a unique fingerprint.

Cite this