Isolation of synaptosomes from the rat urinary bladder

Yat-Ching Tong, Ying Cho Hung, Shinn Nan Lin, Juei Tang Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Synaptosomes are nerve-end particles (NEP) isolated by using the technique of differential centrifugation. The synaptosome offers a good model for biochemical and pharmacological studies of the nerve endings. No report has been made on synaptosome isolation from the urinary bladder, The purpose of our work was to develop the use of synaptosome in the research of neurophysiology and neuropharmacology of the urinary bladder. Synaptosome-rich fraction was prepared from tissue homogenate of male Wistar rat urinary bladder by differential centrifugation (1000, 17,000 and 100,000 g) with discontinuous sucrose gradient. Electron microscopy showed synaptosomes as thin-walled bags containing a large number of synaptic vesicles. Two types of synaptosomes were easily discerned: those containing small agranular vesicles, and those containing dense-cored vesicles. The acetylcholine, norepinephrine, epinephrine and dopamine contents in the preparation were measured by the method of high-performance liquid chromatography. The respective concentrations were 300.4 ± 30.1, 962.8 ± 58.5, 617.3 ± 59.8 and 1354.8 ± 144.2 pmol/mg synaptosomal protein. In conclusion, it has been demonstrated that synaptosome-rich fractions can be prepared from the rat urinary bladder. Thus it is possible to apply this methodology for the investigation of the neurobiology of urinary bladders.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalJournal of the Autonomic Nervous System
Volume58
Issue number1-2
DOIs
Publication statusPublished - 1996 Apr 20

Fingerprint

Synaptosomes
Urinary Bladder
Centrifugation
Neuropharmacology
Neurophysiology
Nerve Endings
Neurobiology
Synaptic Vesicles
Epinephrine
Acetylcholine
Sucrose
Wistar Rats
Dopamine
Norepinephrine
Electron Microscopy
High Pressure Liquid Chromatography
Pharmacology
Research

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Clinical Neurology

Cite this

Tong, Yat-Ching ; Hung, Ying Cho ; Lin, Shinn Nan ; Cheng, Juei Tang. / Isolation of synaptosomes from the rat urinary bladder. In: Journal of the Autonomic Nervous System. 1996 ; Vol. 58, No. 1-2. pp. 76-80.
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Isolation of synaptosomes from the rat urinary bladder. / Tong, Yat-Ching; Hung, Ying Cho; Lin, Shinn Nan; Cheng, Juei Tang.

In: Journal of the Autonomic Nervous System, Vol. 58, No. 1-2, 20.04.1996, p. 76-80.

Research output: Contribution to journalArticle

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