Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

Tomotsugu Koyama; Takashi Okada; Sakihito Kitajima; Masaru Ohme-Takagi; Hideaki Shinshi; Fumihiko Sato

doi:10.1093/jxb/erg136

Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

Tomotsugu Koyama
, Takashi Okada
, Sakihito Kitajima
, Masaru Ohme-Takagi
, Hideaki Shinshi
, Fumihiko Sato

Institute of Tropical Plant Sciences and Microbiology

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (B8) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3.

Original language	English
Pages (from-to)	1175-1181
Number of pages	7
Journal	Journal of experimental botany
Volume	54
Issue number	385
DOIs	https://doi.org/10.1093/jxb/erg136
Publication status	Published - 2003 Apr 1

All Science Journal Classification (ASJC) codes

Physiology
Plant Science

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10.1093/jxb/erg136

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@article{4072a81309b24f0d94a1477d4b8702cb,

title = "Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction",

abstract = "Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (B8) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3.",

author = "Tomotsugu Koyama and Takashi Okada and Sakihito Kitajima and Masaru Ohme-Takagi and Hideaki Shinshi and Fumihiko Sato",

note = "Funding Information: We thank Dr Gregory Martin for a kind gift of Pto cDNA. We are grateful to Drs Kaoru Suzuki and Masaru Ohta for fruitful discussions. This research was supported in part by Grant-in-aid for Research for the Future Program Grant JSPS-RFTF00L01606 from the Japan Society for Promotion of Science (to FS) and a JSPS Fellowship for Japanese Junior Scientists (to TK). The accession numbers are NtUBC1 (AB026055), NtUBC2 (AB026056).",

year = "2003",

month = apr,

day = "1",

doi = "10.1093/jxb/erg136",

language = "English",

volume = "54",

pages = "1175--1181",

journal = "Journal of experimental botany",

issn = "0022-0957",

publisher = "Oxford University Press",

number = "385",

}

Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction. / Koyama, Tomotsugu; Okada, Takashi; Kitajima, Sakihito et al.
In: Journal of experimental botany, Vol. 54, No. 385, 01.04.2003, p. 1175-1181.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

AU - Koyama, Tomotsugu

AU - Okada, Takashi

AU - Kitajima, Sakihito

AU - Ohme-Takagi, Masaru

AU - Shinshi, Hideaki

AU - Sato, Fumihiko

N1 - Funding Information: We thank Dr Gregory Martin for a kind gift of Pto cDNA. We are grateful to Drs Kaoru Suzuki and Masaru Ohta for fruitful discussions. This research was supported in part by Grant-in-aid for Research for the Future Program Grant JSPS-RFTF00L01606 from the Japan Society for Promotion of Science (to FS) and a JSPS Fellowship for Japanese Junior Scientists (to TK). The accession numbers are NtUBC1 (AB026055), NtUBC2 (AB026056).

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (B8) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3.

AB - Tobacco ETHYLENE-RESPONSIVE FACTOR3 (ERF3) is a member of the ERF-domain transcription factors and has a transcriptional repressor activity, whereas other ERF proteins show activation activity. To understand the regulation of ERF3-repressor activity, protein(s) were screened which interact with ERF3 in a yeast two-hybrid system. A partial sequence (B8) of NtUBC2, a tobacco ubiquitin-conjugating enzyme was isolated. This B8 specifically interacted with ERF3 in the yeast two-hybrid system. Further analyses revealed that the region unique to ERF3 interacted with B8. The physiological functions of NtUBC2 and the stability of ERF3 are discussed in relation to the regulation of the repression activity of ERF3.

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ER -

Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction

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