KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The autosomal recessive Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC) is associated with the dysfunction of the K+–Cl cotransporter type 3 (KCC3), which is an electroneutral cotransporter. We previously found that the inhibition of KCC3 cotransporter activity reduces the propagation of action potentials in the peripheral nervous system (PNS). However, the pathogenesis by which KCC3 deficiency impairs peripheral nerve function remains to be examined. Thus, we conducted imaging and electrophysiological studies in the peripheral nerves of KCC3−/− mice at various ages. Analysis using transmission electron microscopy (TEM) revealed an age-dependent progressive swelling of microvilli and disorganization of paranodal loops in KCC3−/− nerves. Yet, no mislocated voltage-dependent channels were observed between the nodes and juxtaparanodes of KCC3−/− nerves. However, electrophysiological studies using the threshold tracking technique indicated a reduced stimulus–response curve slope with an elevated rheobase, a decreased strength–duration time constant, diminished persistent Na+ currents, and an outward deviation of threshold electrotonus in KCC3−/− nerves compared to wild-type nerves. These functional changes indicate an overall reduction in axonal excitability and suggest an increase in paranodal conductance, which was relevant to the pathology at the paranode. Altogether, our findings highlight the importance of KCC3 in maintaining paranodal integrity and in optimizing the propagation of action potentials along peripheral nerves.

Original languageEnglish
Pages (from-to)91-102
Number of pages12
JournalNeuroscience
Volume335
DOIs
Publication statusPublished - 2016 Oct 29

Fingerprint

Peripheral Nervous System
Peripheral Nerves
Action Potentials
potassium-chloride symporters
Microvilli
Transmission Electron Microscopy
Pathology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{0c610e23e1b44a7d9507f6261786e2ce,
title = "KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system",
abstract = "The autosomal recessive Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC) is associated with the dysfunction of the K+–Cl− cotransporter type 3 (KCC3), which is an electroneutral cotransporter. We previously found that the inhibition of KCC3 cotransporter activity reduces the propagation of action potentials in the peripheral nervous system (PNS). However, the pathogenesis by which KCC3 deficiency impairs peripheral nerve function remains to be examined. Thus, we conducted imaging and electrophysiological studies in the peripheral nerves of KCC3−/− mice at various ages. Analysis using transmission electron microscopy (TEM) revealed an age-dependent progressive swelling of microvilli and disorganization of paranodal loops in KCC3−/− nerves. Yet, no mislocated voltage-dependent channels were observed between the nodes and juxtaparanodes of KCC3−/− nerves. However, electrophysiological studies using the threshold tracking technique indicated a reduced stimulus–response curve slope with an elevated rheobase, a decreased strength–duration time constant, diminished persistent Na+ currents, and an outward deviation of threshold electrotonus in KCC3−/− nerves compared to wild-type nerves. These functional changes indicate an overall reduction in axonal excitability and suggest an increase in paranodal conductance, which was relevant to the pathology at the paranode. Altogether, our findings highlight the importance of KCC3 in maintaining paranodal integrity and in optimizing the propagation of action potentials along peripheral nerves.",
author = "Yuan-Ting Sun and Shun-Fen Tzeng and Thy-Sheng Lin and Kuei-Sen Hsu and Eric Delpire and Meng-Ru Shen",
year = "2016",
month = "10",
day = "29",
doi = "10.1016/j.neuroscience.2016.08.031",
language = "English",
volume = "335",
pages = "91--102",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system

AU - Sun, Yuan-Ting

AU - Tzeng, Shun-Fen

AU - Lin, Thy-Sheng

AU - Hsu, Kuei-Sen

AU - Delpire, Eric

AU - Shen, Meng-Ru

PY - 2016/10/29

Y1 - 2016/10/29

N2 - The autosomal recessive Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC) is associated with the dysfunction of the K+–Cl− cotransporter type 3 (KCC3), which is an electroneutral cotransporter. We previously found that the inhibition of KCC3 cotransporter activity reduces the propagation of action potentials in the peripheral nervous system (PNS). However, the pathogenesis by which KCC3 deficiency impairs peripheral nerve function remains to be examined. Thus, we conducted imaging and electrophysiological studies in the peripheral nerves of KCC3−/− mice at various ages. Analysis using transmission electron microscopy (TEM) revealed an age-dependent progressive swelling of microvilli and disorganization of paranodal loops in KCC3−/− nerves. Yet, no mislocated voltage-dependent channels were observed between the nodes and juxtaparanodes of KCC3−/− nerves. However, electrophysiological studies using the threshold tracking technique indicated a reduced stimulus–response curve slope with an elevated rheobase, a decreased strength–duration time constant, diminished persistent Na+ currents, and an outward deviation of threshold electrotonus in KCC3−/− nerves compared to wild-type nerves. These functional changes indicate an overall reduction in axonal excitability and suggest an increase in paranodal conductance, which was relevant to the pathology at the paranode. Altogether, our findings highlight the importance of KCC3 in maintaining paranodal integrity and in optimizing the propagation of action potentials along peripheral nerves.

AB - The autosomal recessive Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC) is associated with the dysfunction of the K+–Cl− cotransporter type 3 (KCC3), which is an electroneutral cotransporter. We previously found that the inhibition of KCC3 cotransporter activity reduces the propagation of action potentials in the peripheral nervous system (PNS). However, the pathogenesis by which KCC3 deficiency impairs peripheral nerve function remains to be examined. Thus, we conducted imaging and electrophysiological studies in the peripheral nerves of KCC3−/− mice at various ages. Analysis using transmission electron microscopy (TEM) revealed an age-dependent progressive swelling of microvilli and disorganization of paranodal loops in KCC3−/− nerves. Yet, no mislocated voltage-dependent channels were observed between the nodes and juxtaparanodes of KCC3−/− nerves. However, electrophysiological studies using the threshold tracking technique indicated a reduced stimulus–response curve slope with an elevated rheobase, a decreased strength–duration time constant, diminished persistent Na+ currents, and an outward deviation of threshold electrotonus in KCC3−/− nerves compared to wild-type nerves. These functional changes indicate an overall reduction in axonal excitability and suggest an increase in paranodal conductance, which was relevant to the pathology at the paranode. Altogether, our findings highlight the importance of KCC3 in maintaining paranodal integrity and in optimizing the propagation of action potentials along peripheral nerves.

UR - http://www.scopus.com/inward/record.url?scp=84984823802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84984823802&partnerID=8YFLogxK

U2 - 10.1016/j.neuroscience.2016.08.031

DO - 10.1016/j.neuroscience.2016.08.031

M3 - Article

VL - 335

SP - 91

EP - 102

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

ER -