Key Role of a CCAAT Element in Regulating Hepatitis B Virus Surface Protein Expression

Cheng Chan Lu, Min Chen, Jing Hsiung Ou, T. S.Benedict Yen

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Two separate promoters, the upstream preS1 and the downstream S promoters, give rise to transcripts encoding three forms of the hepatitis B virus surface protein. Overproduction of large surface protein because of increased preS1 transcripts leads to a block in secretion of all forms of the surface protein and of virion particles. We show here that a CCAAT element in the S promoter not only increases the amount of S transcripts, but also decreases the amount of preS1 transcripts by up to fivefold. Consequently, mutations in this element cause intracellular accumulation of surface proteins because of the secretory block. Therefore, this CCAAT element appears to be critical for maintaining the high ratio of S versus preS1 transcripts that is necessary for the viral life cycle.

Original languageEnglish
Article number71042
Pages (from-to)1155-1158
Number of pages4
JournalVirology
Volume206
Issue number2
DOIs
Publication statusPublished - 1995 Feb 1

All Science Journal Classification (ASJC) codes

  • Virology

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