Kinetic Studies on Oxidation of Allyl Alcohol by Yeast Alcohol Dehydrogenase

T. C. Huang; F. Y. Ke; D. H. Chen

doi:10.1080/00986449508936330

Kinetic Studies on Oxidation of Allyl Alcohol by Yeast Alcohol Dehydrogenase

T. C. Huang, F. Y. Ke, D. H. Chen

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.

Original language	English
Pages (from-to)	171-182
Number of pages	12
Journal	Chemical Engineering Communications
Volume	134
Issue number	1
DOIs	https://doi.org/10.1080/00986449508936330
Publication status	Published - 1995 May 1

All Science Journal Classification (ASJC) codes

General Chemistry
General Chemical Engineering

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10.1080/00986449508936330

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title = "Kinetic Studies on Oxidation of Allyl Alcohol by Yeast Alcohol Dehydrogenase",

abstract = "The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.",

author = "Huang, {T. C.} and Ke, {F. Y.} and Chen, {D. H.}",

note = "Funding Information: This work was performed under the auspices of the National Science Council of the Republic of China, under Contract No. NSC81-0402·E006·14, to which the authors express their thanks.",

year = "1995",

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doi = "10.1080/00986449508936330",

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AU - Huang, T. C.

AU - Ke, F. Y.

AU - Chen, D. H.

N1 - Funding Information: This work was performed under the auspices of the National Science Council of the Republic of China, under Contract No. NSC81-0402·E006·14, to which the authors express their thanks.

PY - 1995/5/1

Y1 - 1995/5/1

N2 - The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.

AB - The kinetics of the oxidation of ally! alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within the YADH concentration range of0-16.4mg/dm3, and YADH exhibited maximum activity at pH 7.9 and a phosphate buffer concentration of 0.25 mol/dm3. The oxidation rate of allyl alcohol by YADH followed the Arrhenius equation within the temperature range of 10 to 40°C. The YADH was denatured irreversibly at the temperatures above 40°C. The rate equation for the YADH-catalyzed oxidation of allyl alcohol was obtained. A mixed-type substrate inhibition occurred when the concentration of allyl alcohol was higher than 200 mmol/dm3. The presence of LiCl, NaCl and KC1 could enhance the activity of YADH. The YADH lost activity significantly in the presence of organic solvents, particularly benzene and its derivatives.

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Kinetic Studies on Oxidation of Allyl Alcohol by Yeast Alcohol Dehydrogenase

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