Kinetic studies on urea hydrolysis by immobilized urease in a batch squeezer and flow reactor

Ting‐Chia ‐C Huang, Dong-Hwang Chen

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12 Citations (Scopus)

Abstract

The immobilization of urease on the reticulated polyurethane foam, and the kinetic phenomenon of urea hydrolysis by the resulting immobilized urease in both batch squeezer and circulated flow reactors were studied. Urease was immobilized with bovine serum albumin and glutaraldehyde on polyurethane foam support of 7 to 15 μm thickness. The residual apparent activity of urease after immobilization was about 50%. The good hydrodynamic property and flexibility of polyurethane foam were retained in solution after immobilization. A modified biofilm reactor model was used to describe the kinetic phenomenon of urea hydrolysis in both batch squeezer and circulated flow reactors. The characteristic parameters of the reactor model for both bioreactors were obtained by combining the Rosenbrock optimization method, the Rungs–Kutta method, and the Newton–Raphson method. The best‐fit results were in good agreement with the experimental data. This study suggests another application of polyurethane foam in enzyme immobilization and immobilized enzyme reactors, which offers potential for practical applications in various bioreactors. © 1992 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)1203-1209
Number of pages7
JournalBiotechnology and Bioengineering
Volume40
Issue number10
DOIs
Publication statusPublished - 1992 Jan 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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