Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral

De Wei Chang, Meng Ling Hsieh, Yan Min Chen, Tsair-Fuh Lin, Jo-Shu Chang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The effect of an algal metabolite, β-cyclocitral, on the cell integrity of two cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed to various concentrations of β-cyclocitral. Scanning electron microscope (SEM) results indicate that the cells of tested species were greatly altered after being exposed to β-cyclocitral. A flow cytometer coupled with the SYTOX stain and chlorophyll-a auto-fluorescence was used to quantify the effect of β-cyclocitral on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments show that about 5-10mgL -1 of β-cyclocitral for the two M. aeruginosa strains and a much lower concentration, 0.1-0.5mgL -1, for N. palea were needed to cause 15-20% of cells to rupture. When the β-cyclocitral concentration was increased to 200-1000mgL -1 for M. aeruginosa and 5-10mgL -1 for N. palea, almost all the cells ruptured between 8 and 24h. A first-order kinetic model is able to describe the data of cell integrity over time. The extracted rate constant values well correlate with the applied β-cyclocitral dosages. The obtained kinetic parameters may be used to estimate β-cyclocitral dosage and contact time required for the control of cyanobacteria and diatoms in water bodies.

Original languageEnglish
Pages (from-to)1214-1220
Number of pages7
JournalJournal of Hazardous Materials
Volume185
Issue number2-3
DOIs
Publication statusPublished - 2011 Jan 30

Fingerprint

Microcystis
lysis
cyanobacterium
Diatoms
diatom
Cells
Cyanobacteria
kinetics
Kinetics
Chlorophyll
Metabolites
Kinetic parameters
rupture
Rate constants
metabolite
chlorophyll a
Coloring Agents
fluorescence
Electron microscopes
Body Water

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

Cite this

@article{2b00ca9edc6d4fa990d41932f68c0a51,
title = "Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral",
abstract = "The effect of an algal metabolite, β-cyclocitral, on the cell integrity of two cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed to various concentrations of β-cyclocitral. Scanning electron microscope (SEM) results indicate that the cells of tested species were greatly altered after being exposed to β-cyclocitral. A flow cytometer coupled with the SYTOX stain and chlorophyll-a auto-fluorescence was used to quantify the effect of β-cyclocitral on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments show that about 5-10mgL -1 of β-cyclocitral for the two M. aeruginosa strains and a much lower concentration, 0.1-0.5mgL -1, for N. palea were needed to cause 15-20{\%} of cells to rupture. When the β-cyclocitral concentration was increased to 200-1000mgL -1 for M. aeruginosa and 5-10mgL -1 for N. palea, almost all the cells ruptured between 8 and 24h. A first-order kinetic model is able to describe the data of cell integrity over time. The extracted rate constant values well correlate with the applied β-cyclocitral dosages. The obtained kinetic parameters may be used to estimate β-cyclocitral dosage and contact time required for the control of cyanobacteria and diatoms in water bodies.",
author = "Chang, {De Wei} and Hsieh, {Meng Ling} and Chen, {Yan Min} and Tsair-Fuh Lin and Jo-Shu Chang",
year = "2011",
month = "1",
day = "30",
doi = "10.1016/j.jhazmat.2010.10.033",
language = "English",
volume = "185",
pages = "1214--1220",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",
number = "2-3",

}

Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral. / Chang, De Wei; Hsieh, Meng Ling; Chen, Yan Min; Lin, Tsair-Fuh; Chang, Jo-Shu.

In: Journal of Hazardous Materials, Vol. 185, No. 2-3, 30.01.2011, p. 1214-1220.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Kinetics of cell lysis for Microcystis aeruginosa and Nitzschia palea in the exposure to β-cyclocitral

AU - Chang, De Wei

AU - Hsieh, Meng Ling

AU - Chen, Yan Min

AU - Lin, Tsair-Fuh

AU - Chang, Jo-Shu

PY - 2011/1/30

Y1 - 2011/1/30

N2 - The effect of an algal metabolite, β-cyclocitral, on the cell integrity of two cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed to various concentrations of β-cyclocitral. Scanning electron microscope (SEM) results indicate that the cells of tested species were greatly altered after being exposed to β-cyclocitral. A flow cytometer coupled with the SYTOX stain and chlorophyll-a auto-fluorescence was used to quantify the effect of β-cyclocitral on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments show that about 5-10mgL -1 of β-cyclocitral for the two M. aeruginosa strains and a much lower concentration, 0.1-0.5mgL -1, for N. palea were needed to cause 15-20% of cells to rupture. When the β-cyclocitral concentration was increased to 200-1000mgL -1 for M. aeruginosa and 5-10mgL -1 for N. palea, almost all the cells ruptured between 8 and 24h. A first-order kinetic model is able to describe the data of cell integrity over time. The extracted rate constant values well correlate with the applied β-cyclocitral dosages. The obtained kinetic parameters may be used to estimate β-cyclocitral dosage and contact time required for the control of cyanobacteria and diatoms in water bodies.

AB - The effect of an algal metabolite, β-cyclocitral, on the cell integrity of two cyanobacteria and one diatom was investigated. The cyanobacteria, Microcystis aeruginosa PCC 7005 and PCC 7820, and the diatom, Nitzschia palea, were exposed to various concentrations of β-cyclocitral. Scanning electron microscope (SEM) results indicate that the cells of tested species were greatly altered after being exposed to β-cyclocitral. A flow cytometer coupled with the SYTOX stain and chlorophyll-a auto-fluorescence was used to quantify the effect of β-cyclocitral on cell integrity for the tested cyanobacteria and diatom. Kinetic experiments show that about 5-10mgL -1 of β-cyclocitral for the two M. aeruginosa strains and a much lower concentration, 0.1-0.5mgL -1, for N. palea were needed to cause 15-20% of cells to rupture. When the β-cyclocitral concentration was increased to 200-1000mgL -1 for M. aeruginosa and 5-10mgL -1 for N. palea, almost all the cells ruptured between 8 and 24h. A first-order kinetic model is able to describe the data of cell integrity over time. The extracted rate constant values well correlate with the applied β-cyclocitral dosages. The obtained kinetic parameters may be used to estimate β-cyclocitral dosage and contact time required for the control of cyanobacteria and diatoms in water bodies.

UR - http://www.scopus.com/inward/record.url?scp=78649917914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649917914&partnerID=8YFLogxK

U2 - 10.1016/j.jhazmat.2010.10.033

DO - 10.1016/j.jhazmat.2010.10.033

M3 - Article

VL - 185

SP - 1214

EP - 1220

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

IS - 2-3

ER -