Kringle domains and plasmin denaturation

Guey Yueh Shi, Dung Ho Wu, Hua Lin Wu

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

The rate of plasmin denaturation was in the order of Lys-plasmin > miniplasmin > microplasmin. Fibrinogen degradation products (FDP) dose dependently increased the denaturation rate of Lys-plasmin and miniplasmin with a maximal rate constant at the FDP/plasmin ratio of about 0.5. The denaturation rate constant of microplasmin was not affected. FDP increased the rate of plasmin denaturation was in parallel with its effect on the interaction among kringle domains. Without FDP only trace amounts of plasminogen dimer could be detected by cross-linking with bis-(sulfosuccinimidyl)-suberate followed by SDS gel electrophoresis. In the low concentration of FDP significant amounts of oligomers of Glu-, miniplasminogens, kringle 1-3 and kringle 1-5 were observed. High concentration of FDP, however, decreased plasminogen oligomer.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume178
Issue number1
DOIs
Publication statusPublished - 1991 Jul 15

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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