TY - JOUR
T1 - Kringle domains and plasmin denaturation
AU - Shi, Guey Yueh
AU - Wu, Dung Ho
AU - Wu, Hua Lin
N1 - Funding Information:
Acknowledgments: The expert technical assistance of L. -C. Chang and G. -J. Hsieh are gratefully acknowledged. This work was supported by Grant NSC 79-0412-B006-48, NSC 79-0412-B006-13, and NSC 80-0412-BOO6-12 from the National Science Council, Republic China.
PY - 1991/7/15
Y1 - 1991/7/15
N2 - The rate of plasmin denaturation was in the order of Lys-plasmin > miniplasmin > microplasmin. Fibrinogen degradation products (FDP) dose dependently increased the denaturation rate of Lys-plasmin and miniplasmin with a maximal rate constant at the FDP/plasmin ratio of about 0.5. The denaturation rate constant of microplasmin was not affected. FDP increased the rate of plasmin denaturation was in parallel with its effect on the interaction among kringle domains. Without FDP only trace amounts of plasminogen dimer could be detected by cross-linking with bis-(sulfosuccinimidyl)-suberate followed by SDS gel electrophoresis. In the low concentration of FDP significant amounts of oligomers of Glu-, miniplasminogens, kringle 1-3 and kringle 1-5 were observed. High concentration of FDP, however, decreased plasminogen oligomer.
AB - The rate of plasmin denaturation was in the order of Lys-plasmin > miniplasmin > microplasmin. Fibrinogen degradation products (FDP) dose dependently increased the denaturation rate of Lys-plasmin and miniplasmin with a maximal rate constant at the FDP/plasmin ratio of about 0.5. The denaturation rate constant of microplasmin was not affected. FDP increased the rate of plasmin denaturation was in parallel with its effect on the interaction among kringle domains. Without FDP only trace amounts of plasminogen dimer could be detected by cross-linking with bis-(sulfosuccinimidyl)-suberate followed by SDS gel electrophoresis. In the low concentration of FDP significant amounts of oligomers of Glu-, miniplasminogens, kringle 1-3 and kringle 1-5 were observed. High concentration of FDP, however, decreased plasminogen oligomer.
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U2 - 10.1016/0006-291X(91)91822-T
DO - 10.1016/0006-291X(91)91822-T
M3 - Article
C2 - 1829887
AN - SCOPUS:0025776956
VL - 178
SP - 360
EP - 368
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -