La 3+ Inhibits the UTP-induced Ca 2+ Mobilization in MDCK Cells

Chung Ren Jan, Chin Man Ho, Sheng-Nan Wu, Jong Khing Huang, Ching Jiunn Tseng

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have studied the effects of La 3+ on UTP-induced rises in intracellular calcium levels ([Ca 2+ ] i ) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. UTP induced [Ca 2+ ] i rises dose-dependently with an EC 50 of 1 mM. The Ca 2+ signal was triggered by a Ca 2+ release from the inositol 1,4,5-trisphosphate (IP 3 )-sensitive pool because the signal was completely blocked by pretreatment of the endoplasmic reticulum (ER) Ca 2+ pump inhibitor thapsigargin (TG) or the phospholipase C (PLC) inhibitor U73122. Both the peak height and under-curve area of 10 μM UTP-induced Ca 2+ signal was reduced by approximately 40% by extracellular Ca 2+ removal, suggesting that UTP induced capacitative Ca 2+ entry. La 3+ inhibited the UTP-induced Ca 2+ signal dose-dependently when added before or after UTP. Pretreatment of 0.1 mM La 3+ inhibited the UTP response more than Ca 2+ removal did. The mechanisms underlying the La 3+ inhibition appear to involve not only block of capacitative Ca 2+ entry.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalChinese Journal of Physiology
Volume41
Issue number1
Publication statusPublished - 1998 Mar 31

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Uridine Triphosphate
Madin Darby Canine Kidney Cells
Fluorometry
Inositol 1,4,5-Trisphosphate
Thapsigargin
Fura-2
Type C Phospholipases
Endoplasmic Reticulum
Area Under Curve
Calcium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Jan, C. R., Ho, C. M., Wu, S-N., Huang, J. K., & Tseng, C. J. (1998). La 3+ Inhibits the UTP-induced Ca 2+ Mobilization in MDCK Cells Chinese Journal of Physiology, 41(1), 59-64.
Jan, Chung Ren ; Ho, Chin Man ; Wu, Sheng-Nan ; Huang, Jong Khing ; Tseng, Ching Jiunn. / La 3+ Inhibits the UTP-induced Ca 2+ Mobilization in MDCK Cells In: Chinese Journal of Physiology. 1998 ; Vol. 41, No. 1. pp. 59-64.
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abstract = "We have studied the effects of La 3+ on UTP-induced rises in intracellular calcium levels ([Ca 2+ ] i ) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. UTP induced [Ca 2+ ] i rises dose-dependently with an EC 50 of 1 mM. The Ca 2+ signal was triggered by a Ca 2+ release from the inositol 1,4,5-trisphosphate (IP 3 )-sensitive pool because the signal was completely blocked by pretreatment of the endoplasmic reticulum (ER) Ca 2+ pump inhibitor thapsigargin (TG) or the phospholipase C (PLC) inhibitor U73122. Both the peak height and under-curve area of 10 μM UTP-induced Ca 2+ signal was reduced by approximately 40{\%} by extracellular Ca 2+ removal, suggesting that UTP induced capacitative Ca 2+ entry. La 3+ inhibited the UTP-induced Ca 2+ signal dose-dependently when added before or after UTP. Pretreatment of 0.1 mM La 3+ inhibited the UTP response more than Ca 2+ removal did. The mechanisms underlying the La 3+ inhibition appear to involve not only block of capacitative Ca 2+ entry.",
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Jan, CR, Ho, CM, Wu, S-N, Huang, JK & Tseng, CJ 1998, ' La 3+ Inhibits the UTP-induced Ca 2+ Mobilization in MDCK Cells ', Chinese Journal of Physiology, vol. 41, no. 1, pp. 59-64.

La 3+ Inhibits the UTP-induced Ca 2+ Mobilization in MDCK Cells . / Jan, Chung Ren; Ho, Chin Man; Wu, Sheng-Nan; Huang, Jong Khing; Tseng, Ching Jiunn.

In: Chinese Journal of Physiology, Vol. 41, No. 1, 31.03.1998, p. 59-64.

Research output: Contribution to journalArticle

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