Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil

Sheng Shung Cheng, Pao Wen Grace Liu, Yi Lei Tzeng, Chih Chiang Ma, Liang-Ming Whang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A study on the potential applicability of bioaugmentation and biostimulation for a chronically diesel-contaminated site was carried out. The results of the bioaugmentation with diesel-degrading bacteria consortium conveyed the following: with the single strain isolate, JG39 and CF3 along reached the highest degradation; with the consortium that combined the strain(s) with the indigenous, [M + CF3] achieved 100% degradation, followed by [M + 4 strains] and [M + JG39] with ∼ 95% degradability. Either CF3 or JG39 along, or both contemporaneously introducing to the indigenous, is a useful strategy recommended to enhance bioremediation of diesel contamination. LB medium was more advanced than MS medium in culturing the Rhamnolipid (RL) producer P. aeruginosa J4. LB medium, indirectly, helped to stimulate the biomass growth by 3.4 folds over the non-RL-addition after the 84-hr inoculation. The optimal operation condition with the RL addition included using LB medium for culturing the RL producer Pseudomonas aeruginosa J4 and pH value controlling at 7.2. This is an abstract of a paper presented at the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005).

Original languageEnglish
Title of host publicationProceedings of the 8th International In Situ and On-Site Bioremediation Symposium
Pages274-281
Number of pages8
Publication statusPublished - 2005 Dec 1
Event8th International In Situ and On-Site Bioremediation Symposium - Baltimore, MD, United States
Duration: 2005 Jun 62005 Jun 9

Publication series

NameProceedings of the 8th International In Situ and On-Site Bioremediation Symposium
Volume1

Other

Other8th International In Situ and On-Site Bioremediation Symposium
CountryUnited States
CityBaltimore, MD
Period05-06-0605-06-09

Fingerprint

Bioremediation
Soils
Degradation
Bacteria
Biomass
Contamination

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Cheng, S. S., Liu, P. W. G., Tzeng, Y. L., Ma, C. C., & Whang, L-M. (2005). Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil. In Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium (pp. 274-281). (Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium; Vol. 1).
Cheng, Sheng Shung ; Liu, Pao Wen Grace ; Tzeng, Yi Lei ; Ma, Chih Chiang ; Whang, Liang-Ming. / Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil. Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium. 2005. pp. 274-281 (Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium).
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title = "Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil",
abstract = "A study on the potential applicability of bioaugmentation and biostimulation for a chronically diesel-contaminated site was carried out. The results of the bioaugmentation with diesel-degrading bacteria consortium conveyed the following: with the single strain isolate, JG39 and CF3 along reached the highest degradation; with the consortium that combined the strain(s) with the indigenous, [M + CF3] achieved 100{\%} degradation, followed by [M + 4 strains] and [M + JG39] with ∼ 95{\%} degradability. Either CF3 or JG39 along, or both contemporaneously introducing to the indigenous, is a useful strategy recommended to enhance bioremediation of diesel contamination. LB medium was more advanced than MS medium in culturing the Rhamnolipid (RL) producer P. aeruginosa J4. LB medium, indirectly, helped to stimulate the biomass growth by 3.4 folds over the non-RL-addition after the 84-hr inoculation. The optimal operation condition with the RL addition included using LB medium for culturing the RL producer Pseudomonas aeruginosa J4 and pH value controlling at 7.2. This is an abstract of a paper presented at the 8th International In Situ and On-Site Bioremediation Symposium (Baltimore, MD 6/6-9/2005).",
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Cheng, SS, Liu, PWG, Tzeng, YL, Ma, CC & Whang, L-M 2005, Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil. in Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium. Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium, vol. 1, pp. 274-281, 8th International In Situ and On-Site Bioremediation Symposium, Baltimore, MD, United States, 05-06-06.

Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil. / Cheng, Sheng Shung; Liu, Pao Wen Grace; Tzeng, Yi Lei; Ma, Chih Chiang; Whang, Liang-Ming.

Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium. 2005. p. 274-281 (Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Cheng SS, Liu PWG, Tzeng YL, Ma CC, Whang L-M. Laboratory-scale bioaugmentation and biostimulation in diesel-contaminated soil. In Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium. 2005. p. 274-281. (Proceedings of the 8th International In Situ and On-Site Bioremediation Symposium).