Lactoferrin inhibits enterovirus 71 infection by binding to VP1 protein and host cells

Tsui Ying Weng, Lien Cheng Chen, Huey Wen Shyu, Shun Hua Chen, Jen Ren Wang, Chun Keung Yu, Huan Yao Lei, Trai Ming Yeh

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

The antiviral activities of bovine lactoferrin (LF) against enterovirus 71 (EV71) were studied both in vitro and in vivo. LF protected both human rhabdomyosarcoma and neuroblastoma SK-N-SH cell lines from EV71 infection when it was added at the same time, before, or within 30 min after EV71 infection. Using enzyme-linked immunosorbent assay-based binding assay and indirect fluorescent stain, we found that LF could bind to the target cells. Furthermore, it was found that LF could bind to the VP1 protein of EV71, which was blocked in the presence of anti-VP1 antibody. In addition, LF could induce IFN-α expression of SK-N-SH cells and inhibit EV71-induced IL-6 production. Finally, LF protected mice against lethal EV71 challenge. Taken together, these results suggest that LF can inhibit EV71 infection by interacting with both EV71 and host cells.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalAntiviral Research
Volume67
Issue number1
DOIs
Publication statusPublished - 2005 Jul

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Virology

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