Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

Koichi Toyokura; Tatsuaki Goh; Hidefumi Shinohara; Akinori Shinoda; Yuki Kondo; Yoshie Okamoto; Takeo Uehara; Koichi Fujimoto; Yoko Okushima; Yoshifumi Ikeyama; Keiji Nakajima; Tetsuro Mimura; Masao Tasaka; Yoshikatsu Matsubayashi; Hidehiro Fukaki

doi:10.1016/j.devcel.2018.11.031

Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

Koichi Toyokura, Tatsuaki Goh, Hidefumi Shinohara, Akinori Shinoda, Yuki Kondo, Yoshie Okamoto, Takeo Uehara, Koichi Fujimoto, Yoko Okushima, Yoshifumi Ikeyama, Keiji Nakajima, Tetsuro Mimura, Masao Tasaka, Yoshikatsu Matsubayashi, Hidehiro Fukaki

NCKU-AS Graduate-Program in Translational Agriculture Sciences

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.

Original language	English
Pages (from-to)	64-75.e5
Journal	Developmental Cell
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.devcel.2018.11.031
Publication status	Published - 2019 Jan 7

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry,Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2018.11.031

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Toyokura, K., Goh, T., Shinohara, H., Shinoda, A., Kondo, Y., Okamoto, Y., Uehara, T., Fujimoto, K., Okushima, Y., Ikeyama, Y., Nakajima, K., Mimura, T., Tasaka, M., Matsubayashi, Y., & Fukaki, H. (2019). Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation. Developmental Cell, 48(1), 64-75.e5. https://doi.org/10.1016/j.devcel.2018.11.031

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title = "Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation",

abstract = "In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.",

author = "Koichi Toyokura and Tatsuaki Goh and Hidefumi Shinohara and Akinori Shinoda and Yuki Kondo and Yoshie Okamoto and Takeo Uehara and Koichi Fujimoto and Yoko Okushima and Yoshifumi Ikeyama and Keiji Nakajima and Tetsuro Mimura and Masao Tasaka and Yoshikatsu Matsubayashi and Hidehiro Fukaki",

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Toyokura, K, Goh, T, Shinohara, H, Shinoda, A, Kondo, Y, Okamoto, Y, Uehara, T, Fujimoto, K, Okushima, Y, Ikeyama, Y, Nakajima, K, Mimura, T, Tasaka, M, Matsubayashi, Y & Fukaki, H 2019, 'Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation', Developmental Cell, vol. 48, no. 1, pp. 64-75.e5. https://doi.org/10.1016/j.devcel.2018.11.031

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AU - Toyokura, Koichi

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AU - Shinoda, Akinori

AU - Kondo, Yuki

AU - Okamoto, Yoshie

AU - Uehara, Takeo

AU - Fujimoto, Koichi

AU - Okushima, Yoko

AU - Ikeyama, Yoshifumi

AU - Nakajima, Keiji

AU - Mimura, Tetsuro

AU - Tasaka, Masao

AU - Matsubayashi, Yoshikatsu

AU - Fukaki, Hidehiro

PY - 2019/1/7

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N2 - In plants, the position of lateral roots (LRs) depends on initiation sites induced by auxin. The domain of high auxin response responsible for LR initiation stretches over several cells, but only a pair of pericycle cells (LR founder cells) will develop into LRs. In this work, we identified a signaling cascade controlling LR formation through lateral inhibition. It comprises a peptide hormone TARGET OF LBD SIXTEEN 2 (TOLS2), its receptor RLK7, and a downstream transcription factor PUCHI. TOLS2 is expressed at the LR founder cells and inhibits LR initiation. Time-lapse imaging of auxin-responsive DR5:LUCIFERASE reporter expression revealed that occasionally two pairs of LR founder cells are specified in close proximity even in wild-type and that one of them exists only transiently and disappears in an RLK7-dependent manner. We propose that the selection of LR founder cells by the peptide hormone-receptor cascade ensures proper LR spacing.

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Lateral Inhibition by a Peptide Hormone-Receptor Cascade during Arabidopsis Lateral Root Founder Cell Formation

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