@article{171d3b11255b4eb1a109f4f6c70856ea,
title = "Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana",
abstract = "Lateral root (LR) formation in Arabidopsis thaliana is initiated by asymmetric division of founder cells, followed by coordinated cell proliferation and differentiation for patterning new primordia. The sequential developmental processes of LR formation are triggered by a localized auxin response. LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16), an auxin-inducible transcription factor, is one of the key regulators linking auxin response in LR founder cells to LR initiation. We identified key genes for LR formation that are activated by LBD16 in an auxin-dependent manner. LBD16 targets identified include the transcription factor gene PUCHI, which is required for LR primordium patterning. We demonstrate that LBD16 activity is required for the auxin-inducible expression of PUCHI. We show that PUCHI expression is initiated after the first round of asymmetric cell division of LR founder cells and that premature induction of PUCHI during the preinitiation phase disrupts LR primordium formation. Our results indicate that LR initiation requires the sequential induction of transcription factors LBD16 and PUCHI.",
author = "Tatsuaki Goh and Koichi Toyokura and Nobutoshi Yamaguchi and Yoshie Okamoto and Takeo Uehara and Shutaro Kaneko and Yumiko Takebayashi and Hiroyuki Kasahara and Yoshifumi Ikeyama and Yoko Okushima and Keiji Nakajima and Tetsuro Mimura and Masao Tasaka and Hidehiro Fukaki",
note = "Funding Information: We thank T. Guilfoyle (University of Missouri, USA) for DR5:GUS, P. N. Benfey (Duke University, USA) for DR5:LUC, T. Beeckman (VIB/Ghent University, Belgium) for GATA23pro:NLS-GUS-GFP, T. Nakagawa (Shimane University, Japan) for pGWB vectors, K. Ohashi-Ito and Y. Sugisawa (The University of Tokyo, Japan) for processing raw microarray data, and S. Sera for technical assistance. We thank L. Laplaze (UMR DIADE, Montpellier, France) and M. J. Bennett (The University of Nottingham, UK) for helpful discussion and comments on the manuscript. This work was funded by grants from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan: Grants-in-Aid for Scientific Research on Innovative Areas (25110330 and 25113001 to HF), Grant-in-Aid for Scientific Research on Priority Areas (19060006 to HF), Grant-in-Aid for Scientific Research (B) (18H02463 to HF, 18H02457 to HK) and Grant-in-Aid for Young Scientists (21770045 and 17K15140 to TG). TG and KT were supported by a JSPS Research Fellowship for Young Scientists and JSPS KAKENHI grant nos. 12J02079 and 16J00131, respectively. Publisher Copyright: {\textcopyright} 2019 The Authors. New Phytologist {\textcopyright} 2019 New Phytologist Trust",
year = "2019",
month = oct,
day = "1",
doi = "10.1111/nph.16065",
language = "English",
volume = "224",
pages = "749--760",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "2",
}