Leptin regulates KATPchannel trafficking in pancreatic β-cells by a signaling mechanism involving AMP-activated protein kinase (AMPK) and camp-dependent protein kinase (PKA)

Pei Chun Chen, Yelena N. Kryukova, Show Ling Shyng

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Pancreatic β-cells secrete insulin in response to metabolic and hormonal signals to maintain glucose homeostasis. Insulin secretion is under the control of ATP-sensitive potassium (KATP) channels that play key roles in setting β-cell membrane potential. Leptin, a hormone secreted by adipocytes, inhibits insulin secretion by increasing KATPchannel conductance in β-cells. We investigated the mechanism by which leptin increases KATPchannel conductance. We show that leptin causes a transient increase in surface expression of KATPchannels without affecting channel gating properties. This increase results primarily from increased channel trafficking to the plasma membrane rather than reduced endocytosis of surface channels. The effect of leptin on KATPchannels is dependent on the protein kinases AMP-activated protein kinase (AMPK) and PKA. Activation of AMPK or PKA mimics and inhibition of AMPK or PKA abrogates the effect of leptin. Leptin activates AMPK directly by increasing AMPK phosphorylation at threonine 172. Activation of PKA leads to increased channel surface expression even in the presence of AMPK inhibitors, suggesting AMPK lies upstream of PKA in the leptin signaling pathway. Leptin signaling also leads to F-actin depolymerization. Stabilization of F-actin pharmacologically occludes, whereas destabilization of F-actin simulates, the effect of leptin on K ATPchannel trafficking, indicating that leptin-induced actin reorganization underlies enhanced channel trafficking to the plasma membrane. Our study uncovers the signaling and cellular mechanism by which leptin regulates KATPchannel trafficking to modulate β-cell function and insulin secretion.

Original languageEnglish
Pages (from-to)34098-34109
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number47
DOIs
Publication statusPublished - 2013 Nov 22

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AMP-Activated Protein Kinases
Leptin
Protein Kinases
Actins
Cell membranes
Insulin
KATP Channels
Cell Membrane
Chemical activation
Depolymerization
Phosphorylation
Threonine
Protein Kinase Inhibitors
Endocytosis
Adipocytes
Membrane Potentials
Potassium
Homeostasis
Stabilization
Adenosine Triphosphate

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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title = "Leptin regulates KATPchannel trafficking in pancreatic β-cells by a signaling mechanism involving AMP-activated protein kinase (AMPK) and camp-dependent protein kinase (PKA)",
abstract = "Pancreatic β-cells secrete insulin in response to metabolic and hormonal signals to maintain glucose homeostasis. Insulin secretion is under the control of ATP-sensitive potassium (KATP) channels that play key roles in setting β-cell membrane potential. Leptin, a hormone secreted by adipocytes, inhibits insulin secretion by increasing KATPchannel conductance in β-cells. We investigated the mechanism by which leptin increases KATPchannel conductance. We show that leptin causes a transient increase in surface expression of KATPchannels without affecting channel gating properties. This increase results primarily from increased channel trafficking to the plasma membrane rather than reduced endocytosis of surface channels. The effect of leptin on KATPchannels is dependent on the protein kinases AMP-activated protein kinase (AMPK) and PKA. Activation of AMPK or PKA mimics and inhibition of AMPK or PKA abrogates the effect of leptin. Leptin activates AMPK directly by increasing AMPK phosphorylation at threonine 172. Activation of PKA leads to increased channel surface expression even in the presence of AMPK inhibitors, suggesting AMPK lies upstream of PKA in the leptin signaling pathway. Leptin signaling also leads to F-actin depolymerization. Stabilization of F-actin pharmacologically occludes, whereas destabilization of F-actin simulates, the effect of leptin on K ATPchannel trafficking, indicating that leptin-induced actin reorganization underlies enhanced channel trafficking to the plasma membrane. Our study uncovers the signaling and cellular mechanism by which leptin regulates KATPchannel trafficking to modulate β-cell function and insulin secretion.",
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Leptin regulates KATPchannel trafficking in pancreatic β-cells by a signaling mechanism involving AMP-activated protein kinase (AMPK) and camp-dependent protein kinase (PKA). / Chen, Pei Chun; Kryukova, Yelena N.; Shyng, Show Ling.

In: Journal of Biological Chemistry, Vol. 288, No. 47, 22.11.2013, p. 34098-34109.

Research output: Contribution to journalArticle

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