Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation

Si Yen Liu, Wei Zhi Wang, Chia Liang Yen, Ming Yi Tsai, Pei Wen Yang, Jiu Yao Wang, Chun Yi Ho, Chi Chang Shieh

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background: Isocyanates are low-molecular-weight compounds noted for inducing occupational and environmental asthma. Isocyanate-induced lung disease, an oxidant stress-dependent pulmonary inflammation, is the leading cause of occupational asthma. Objectives: To address the role of leukocyte-produced oxidants in airway inflammation induced by toluene diisocyanate (TDI), and to elucidate the role of leukocyte nicotinamide adenine dinucleotide phosphate-reduced (NADPH) oxidase in pathogenesis by TDI. Methods: Wild-type mice and NADPH oxidase-deficient mice (neutrophil cytosolic factor 1 mutant, Ncf1-/-) were intranasally injected, challenged with inhalatory TDI, and then investigated for lung inflammation. Results: Cell infiltration in lung tissue and leukocytes in bronchoalveolar lavage, airway reactivity to a methacholine challenge, and TDI-induced inflammatory cytokine expression and nuclear factor activation in the lung tissue were all markedly lower in Ncf1-/- mice. Wild-type mice treated with blocking antibodies against CD4 and IL-17 showed markedly lower TDI-induced airway hyperresponsiveness. Conclusion: Leukocyte NADPH oxidase is an essential regulator in TDI-induced airway inflammation through redox modification of immune responses.

Original languageEnglish
Pages (from-to)1014-1023
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume127
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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