TY - JOUR
T1 - Light factors and nitrogen availability to enhance biomass and C-phycocyanin productivity of Thermosynechococcus sp. CL-1
AU - Hoi, Soi Keong
AU - Winayu, Birgitta Narindri Rara
AU - Hsueh, Hsin Ta
AU - Chu, Hsin
N1 - Funding Information:
The authors gratefully thank the National Science Council Taiwan, Taiwan , regarding to the financial support on the research NSC NSC 102-2622-E-006-006-MY3 .
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/3
Y1 - 2021/3
N2 - In this study, the light factors and nitrogen availability effect on Thermosynechococcus sp. CL-1 (TCL-1) productivity of biomass and C-phycocyanin were observed. The variation of light factors (intensity, color and arrangement) and concentration of dissolved inorganic nitrogen (DIN) from 2.9 to 116.7 mM were applied in the TCL-1 cultivation with 3 g/L initial biomass concentration. Moreover, extended cultivation using two DIN concentrations with better results and various lighting arrangements were conducted. Additionally, the malondialdehyde (MDA) content in the biomass was also measured as the indicator of oxidative stress. The double-side lighting arrangement with the intensity of 500 and 1000 μmole m−2 s-1 reach better biomass productivity while the mixed lighting with red LED enhances C-phycocyanin productivity. Outstanding C-phycocyanin productivity of 281.4 ± 10 mg L-1 day-1 is achieved by the application of double side mixed color lighting and the intensity of 250 μmole m−2 s-1 on each side. Application of 2.9 mM DIN in the TCL-1 culture gains the highest biomass productivity recorded as 2022 ± 162 mg L-1day-1. Otherwise, highest C-phycocyanin productivity is 201.6 ± 40.4 mg L-1day-1 with 14.6 mM DIN. The higher concentration of MDA, which indicates the larger amount of free radical in the FPBR, is produced in the cultivation using 2.9 mM DIN.
AB - In this study, the light factors and nitrogen availability effect on Thermosynechococcus sp. CL-1 (TCL-1) productivity of biomass and C-phycocyanin were observed. The variation of light factors (intensity, color and arrangement) and concentration of dissolved inorganic nitrogen (DIN) from 2.9 to 116.7 mM were applied in the TCL-1 cultivation with 3 g/L initial biomass concentration. Moreover, extended cultivation using two DIN concentrations with better results and various lighting arrangements were conducted. Additionally, the malondialdehyde (MDA) content in the biomass was also measured as the indicator of oxidative stress. The double-side lighting arrangement with the intensity of 500 and 1000 μmole m−2 s-1 reach better biomass productivity while the mixed lighting with red LED enhances C-phycocyanin productivity. Outstanding C-phycocyanin productivity of 281.4 ± 10 mg L-1 day-1 is achieved by the application of double side mixed color lighting and the intensity of 250 μmole m−2 s-1 on each side. Application of 2.9 mM DIN in the TCL-1 culture gains the highest biomass productivity recorded as 2022 ± 162 mg L-1day-1. Otherwise, highest C-phycocyanin productivity is 201.6 ± 40.4 mg L-1day-1 with 14.6 mM DIN. The higher concentration of MDA, which indicates the larger amount of free radical in the FPBR, is produced in the cultivation using 2.9 mM DIN.
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U2 - 10.1016/j.bej.2020.107899
DO - 10.1016/j.bej.2020.107899
M3 - Article
AN - SCOPUS:85098108243
VL - 167
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
M1 - 107899
ER -