Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast

Po Lin Yang, Tzu Han Hsu, Chao Wen Wang, Rey Huei Chen

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The neutral lipids steryl ester and triacylglycerol (TAG) are stored in the membrane-bound organelle lipid droplet (LD) in essentially all eukaryotic cells. It is unclear what physiological conditions require the mobilization or storage of these lipids. Here, we study the budding yeast mutant are1Δ are2Δ dga1Δ lro1Δ, which cannot synthesize the neutral lipids and therefore lacks LDs. This quadruple mutant is delayed at cell separation upon release from mitotic arrest. The cells have abnormal septa, unstable septin assembly during cytokinesis, and prolonged exocytosis at the division site at the end of cytokinesis. Lipidomic analysis shows a marked increase of diacylglycerol (DAG) and phosphatidic acid, the precursors for TAG, in the mutant during mitotic exit. The cytokinesis and separation defects are rescued by adding phospholipid precursors or inhibiting fatty acid synthesis, which both reduce DAG levels. Our results suggest that converting excess lipids to neutral lipids for storage during mitotic exit is important for proper execution of cytokinesis and efficient cell separation.

Original languageEnglish
Pages (from-to)2368-2380
Number of pages13
JournalMolecular Biology of the Cell
Volume27
Issue number15
DOIs
Publication statusPublished - 2016 Aug 1

All Science Journal Classification (ASJC) codes

  • General Medicine

Fingerprint

Dive into the research topics of 'Lipid droplets maintain lipid homeostasis during anaphase for efficient cell separation in budding yeast'. Together they form a unique fingerprint.

Cite this