Liposome encapsulation of fluorescent nanoparticles

Quantum dots and silica nanoparticles

Chien-Sheng Chen, Jie Yao, Richard A. Durst

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy was used to measure the average number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. Each liposome encapsulated an average of three QDs. Our work demonstrated that nanoparticles could be successfully encapsulated into liposomes and provided a methodology to measure the nanoparticle quantity inside each liposome with fluorescence correlation spectroscopy.

Original languageEnglish
Title of host publication2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
EditorsM. Laudon, B. Romanowicz
Pages206-209
Number of pages4
Publication statusPublished - 2005 Dec 1
Event2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 - Anaheim, CA, United States
Duration: 2005 May 82005 May 12

Publication series

Name2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings

Other

Other2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005
CountryUnited States
CityAnaheim, CA
Period05-05-0805-05-12

Fingerprint

Liposomes
Encapsulation
Semiconductor quantum dots
Silica
Nanoparticles
Fluorescence
Spectroscopy
Size exclusion chromatography
Fluorophores
Bioassay
Quenching
Gels
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Chen, C-S., Yao, J., & Durst, R. A. (2005). Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles. In M. Laudon, & B. Romanowicz (Eds.), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings (pp. 206-209). (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).
Chen, Chien-Sheng ; Yao, Jie ; Durst, Richard A. / Liposome encapsulation of fluorescent nanoparticles : Quantum dots and silica nanoparticles. 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. editor / M. Laudon ; B. Romanowicz. 2005. pp. 206-209 (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).
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abstract = "Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy was used to measure the average number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. Each liposome encapsulated an average of three QDs. Our work demonstrated that nanoparticles could be successfully encapsulated into liposomes and provided a methodology to measure the nanoparticle quantity inside each liposome with fluorescence correlation spectroscopy.",
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Chen, C-S, Yao, J & Durst, RA 2005, Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles. in M Laudon & B Romanowicz (eds), 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings, pp. 206-209, 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005, Anaheim, CA, United States, 05-05-08.

Liposome encapsulation of fluorescent nanoparticles : Quantum dots and silica nanoparticles. / Chen, Chien-Sheng; Yao, Jie; Durst, Richard A.

2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. ed. / M. Laudon; B. Romanowicz. 2005. p. 206-209 (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Liposome encapsulation of fluorescent nanoparticles

T2 - Quantum dots and silica nanoparticles

AU - Chen, Chien-Sheng

AU - Yao, Jie

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AB - Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes and separated nanoparticle-loaded liposomes from unencapsulated nanoparticles by size exclusion chromatography. Fluorescence correlation spectroscopy was used to measure the average number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. Each liposome encapsulated an average of three QDs. Our work demonstrated that nanoparticles could be successfully encapsulated into liposomes and provided a methodology to measure the nanoparticle quantity inside each liposome with fluorescence correlation spectroscopy.

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Chen C-S, Yao J, Durst RA. Liposome encapsulation of fluorescent nanoparticles: Quantum dots and silica nanoparticles. In Laudon M, Romanowicz B, editors, 2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings. 2005. p. 206-209. (2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings).