Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine.

J. S. Huang, K. M. Liu, C. C. Chen, K. Y. Ho, Y. M. Wu, C. C. Wang, Y. M. Cheng, W. L. Ko, Ching-Hsun Liu, Y. P. Ho, Y. P. Wang, K. Hong

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Abstract

Hydroxyapatite and tricalcium phosphate have been used as bone implants for some period of time. Now unfortunately, these materials have failed to become the nucleation sites for bone regeneration. We hypothesized that coating hydroxyapatite and tricalcium phosphate with negatively charged liposomes may improve the nucleation process for new bone formation. The present study was designed to test this hypothesis. Experiments were carried out in 15 miniature swines' mandibular angle with artificial bony defects. In each of the swine, the bony defects on one side were implanted with either liposomes coated with hydroxyapatite or liposomes coated with tricalcium phosphate, while the other side served as control. At the end of the third and sixth weeks following the operation, we observed result, took histology and radiographs of the operated area. The results showed that liposomes-coated materials were biocompatible and their clinical endpoint was enhanced. At the end of the third week, the implant material was surrounded by dense connective tissues. At the end of the sixth week, there were new bone formations near the implanted material. In addition, liposomes which were immobilized in agarose gel and implanted in the defects showed new bony bridge formation. These observations suggest that liposomes have the ability in promoting repair of osseous deficiencies.

Original languageEnglish
Pages (from-to)213-228
Number of pages16
JournalThe Kaohsiung journal of medical sciences
Volume13
Issue number4
Publication statusPublished - 1997 Jan 1

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Miniature Swine
Durapatite
Liposomes
Biocompatible Coated Materials
Osteogenesis
Bone Regeneration
Connective Tissue
Sepharose
tricalcium phosphate
Histology
Swine
Gels
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Huang, J. S., Liu, K. M., Chen, C. C., Ho, K. Y., Wu, Y. M., Wang, C. C., ... Hong, K. (1997). Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine. The Kaohsiung journal of medical sciences, 13(4), 213-228.
Huang, J. S. ; Liu, K. M. ; Chen, C. C. ; Ho, K. Y. ; Wu, Y. M. ; Wang, C. C. ; Cheng, Y. M. ; Ko, W. L. ; Liu, Ching-Hsun ; Ho, Y. P. ; Wang, Y. P. ; Hong, K. / Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine. In: The Kaohsiung journal of medical sciences. 1997 ; Vol. 13, No. 4. pp. 213-228.
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abstract = "Hydroxyapatite and tricalcium phosphate have been used as bone implants for some period of time. Now unfortunately, these materials have failed to become the nucleation sites for bone regeneration. We hypothesized that coating hydroxyapatite and tricalcium phosphate with negatively charged liposomes may improve the nucleation process for new bone formation. The present study was designed to test this hypothesis. Experiments were carried out in 15 miniature swines' mandibular angle with artificial bony defects. In each of the swine, the bony defects on one side were implanted with either liposomes coated with hydroxyapatite or liposomes coated with tricalcium phosphate, while the other side served as control. At the end of the third and sixth weeks following the operation, we observed result, took histology and radiographs of the operated area. The results showed that liposomes-coated materials were biocompatible and their clinical endpoint was enhanced. At the end of the third week, the implant material was surrounded by dense connective tissues. At the end of the sixth week, there were new bone formations near the implanted material. In addition, liposomes which were immobilized in agarose gel and implanted in the defects showed new bony bridge formation. These observations suggest that liposomes have the ability in promoting repair of osseous deficiencies.",
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Huang, JS, Liu, KM, Chen, CC, Ho, KY, Wu, YM, Wang, CC, Cheng, YM, Ko, WL, Liu, C-H, Ho, YP, Wang, YP & Hong, K 1997, 'Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine.', The Kaohsiung journal of medical sciences, vol. 13, no. 4, pp. 213-228.

Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine. / Huang, J. S.; Liu, K. M.; Chen, C. C.; Ho, K. Y.; Wu, Y. M.; Wang, C. C.; Cheng, Y. M.; Ko, W. L.; Liu, Ching-Hsun; Ho, Y. P.; Wang, Y. P.; Hong, K.

In: The Kaohsiung journal of medical sciences, Vol. 13, No. 4, 01.01.1997, p. 213-228.

Research output: Contribution to journalArticle

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T1 - Liposomes-coated hydroxyapatite and tricalcium phosphate implanted in the mandibular bony defect of miniature swine.

AU - Huang, J. S.

AU - Liu, K. M.

AU - Chen, C. C.

AU - Ho, K. Y.

AU - Wu, Y. M.

AU - Wang, C. C.

AU - Cheng, Y. M.

AU - Ko, W. L.

AU - Liu, Ching-Hsun

AU - Ho, Y. P.

AU - Wang, Y. P.

AU - Hong, K.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Hydroxyapatite and tricalcium phosphate have been used as bone implants for some period of time. Now unfortunately, these materials have failed to become the nucleation sites for bone regeneration. We hypothesized that coating hydroxyapatite and tricalcium phosphate with negatively charged liposomes may improve the nucleation process for new bone formation. The present study was designed to test this hypothesis. Experiments were carried out in 15 miniature swines' mandibular angle with artificial bony defects. In each of the swine, the bony defects on one side were implanted with either liposomes coated with hydroxyapatite or liposomes coated with tricalcium phosphate, while the other side served as control. At the end of the third and sixth weeks following the operation, we observed result, took histology and radiographs of the operated area. The results showed that liposomes-coated materials were biocompatible and their clinical endpoint was enhanced. At the end of the third week, the implant material was surrounded by dense connective tissues. At the end of the sixth week, there were new bone formations near the implanted material. In addition, liposomes which were immobilized in agarose gel and implanted in the defects showed new bony bridge formation. These observations suggest that liposomes have the ability in promoting repair of osseous deficiencies.

AB - Hydroxyapatite and tricalcium phosphate have been used as bone implants for some period of time. Now unfortunately, these materials have failed to become the nucleation sites for bone regeneration. We hypothesized that coating hydroxyapatite and tricalcium phosphate with negatively charged liposomes may improve the nucleation process for new bone formation. The present study was designed to test this hypothesis. Experiments were carried out in 15 miniature swines' mandibular angle with artificial bony defects. In each of the swine, the bony defects on one side were implanted with either liposomes coated with hydroxyapatite or liposomes coated with tricalcium phosphate, while the other side served as control. At the end of the third and sixth weeks following the operation, we observed result, took histology and radiographs of the operated area. The results showed that liposomes-coated materials were biocompatible and their clinical endpoint was enhanced. At the end of the third week, the implant material was surrounded by dense connective tissues. At the end of the sixth week, there were new bone formations near the implanted material. In addition, liposomes which were immobilized in agarose gel and implanted in the defects showed new bony bridge formation. These observations suggest that liposomes have the ability in promoting repair of osseous deficiencies.

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