TY - JOUR
T1 - Liquid triphasic systems as sustainable downstream processing of Chlorella sp. biorefinery for potential biofuels and feed production
AU - Koyande, Apurav Krishna
AU - Chew, Kit Wayne
AU - Show, Pau Loke
AU - Munawaroh, Heli Siti Halimatul
AU - Chang, Jo Shu
N1 - Funding Information:
This work was also supported by the Fundamental Research Grant Scheme, Malaysia [ FRGS/1/2019/STG05/UNIM/02/2 ] and MyPAIR-PHC-Hibiscus Grant [ MyPAIR/1/2020/STG05/UNIM/1 ]. The financial support by the Ministry of Science and Technology, Taiwan , under grant number MOST 109-3116-F-006-016-CC1 , 109-2621-M-029-001 , and 107-2221-E-006-112-MY3 is greatly appreciated.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/8
Y1 - 2021/8
N2 - Microalgae are potential sustainable renewable sources of energy but are highly underutilized due to the expensive and time-consuming downstream processing. This study aims at curbing these obstacles by extracting multiple components with a single processing unit. In this work, an ultrasound-assisted liquid triphasic flotation system was incorporated to extract proteins, lipids, and carbohydrates by phase separation. The parameters involved were optimized and the final recovery efficiency of proteins, lipids, and carbohydrates was determined. A control run involving conventional three-phase partitioning and a 15-fold scale-up system with the recycling of phase components were also performed. Gas Chromatograph and Fourier Transform Infrared spectroscopy were used to examine the potential of extracted products as a source of biofuel. This biorefinery approach is crucial in commercializing microalgae for biodiesel and bioethanol generation with a side product of purified proteins as feed.
AB - Microalgae are potential sustainable renewable sources of energy but are highly underutilized due to the expensive and time-consuming downstream processing. This study aims at curbing these obstacles by extracting multiple components with a single processing unit. In this work, an ultrasound-assisted liquid triphasic flotation system was incorporated to extract proteins, lipids, and carbohydrates by phase separation. The parameters involved were optimized and the final recovery efficiency of proteins, lipids, and carbohydrates was determined. A control run involving conventional three-phase partitioning and a 15-fold scale-up system with the recycling of phase components were also performed. Gas Chromatograph and Fourier Transform Infrared spectroscopy were used to examine the potential of extracted products as a source of biofuel. This biorefinery approach is crucial in commercializing microalgae for biodiesel and bioethanol generation with a side product of purified proteins as feed.
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U2 - 10.1016/j.biortech.2021.125075
DO - 10.1016/j.biortech.2021.125075
M3 - Article
C2 - 33872996
AN - SCOPUS:85104361521
SN - 0960-8524
VL - 333
JO - Bioresource technology
JF - Bioresource technology
M1 - 125075
ER -