Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration

Guo Wei Jheng, Sung Sik Hur, Chia Ming Chang, Chun Chieh Wu, Jia Shing Cheng, Hsiao Hui Lee, Bon Chu Chung, Yang-Gao Wang, Keng Hui Lin, Juan C. del Álamo, Shu Chien, Jin Wu Tsai

Research output: Contribution to journalArticle

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Abstract

Cell migration is a critical process during development, tissue repair, and cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal dynamics, and force generation. Lis1 plays an important role in the migration of neurons, fibroblasts and other cell types, and is essential for normal development of the cerebral cortex. Mutations in human LIS1 gene cause classical lissencephaly (smooth brain), resulting from defects in neuronal migration. However, how Lis1 may affect force generation in migrating cells is still not fully understood. Using traction force microscopy (TFM) with live cell imaging to measure cellular traction force in migrating NIH3T3 cells, we showed that Lis1 knockdown (KD) by RNA interference (RNAi) caused reductions in cell migration and traction force against the extracellular matrix (ECM). Immunostaining of cytoskeletal components in Lis1 KD cells showed disorganization of microtubules and actin filaments. Interestingly, focal adhesions at the cell periphery were significantly reduced. These results suggest that Lis1 is important for cellular traction force generation through the regulation of cytoskeleton organization and focal adhesion formation in migrating cells.

Original languageEnglish
Pages (from-to)869-875
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume497
Issue number3
DOIs
Publication statusPublished - 2018 Mar 11

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Traction
Cell Movement
Adhesion
Cell adhesion
Fibroblasts
Neurons
Actins
Brain
Microscopic examination
Repair
Genes
RNA
Tissue
Focal Adhesions
Imaging techniques
Defects
Lissencephaly
Atomic Force Microscopy
RNA Interference
Cytoskeleton

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Jheng, Guo Wei ; Hur, Sung Sik ; Chang, Chia Ming ; Wu, Chun Chieh ; Cheng, Jia Shing ; Lee, Hsiao Hui ; Chung, Bon Chu ; Wang, Yang-Gao ; Lin, Keng Hui ; del Álamo, Juan C. ; Chien, Shu ; Tsai, Jin Wu. / Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration. In: Biochemical and Biophysical Research Communications. 2018 ; Vol. 497, No. 3. pp. 869-875.
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Jheng, GW, Hur, SS, Chang, CM, Wu, CC, Cheng, JS, Lee, HH, Chung, BC, Wang, Y-G, Lin, KH, del Álamo, JC, Chien, S & Tsai, JW 2018, 'Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration', Biochemical and Biophysical Research Communications, vol. 497, no. 3, pp. 869-875. https://doi.org/10.1016/j.bbrc.2018.02.151

Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration. / Jheng, Guo Wei; Hur, Sung Sik; Chang, Chia Ming; Wu, Chun Chieh; Cheng, Jia Shing; Lee, Hsiao Hui; Chung, Bon Chu; Wang, Yang-Gao; Lin, Keng Hui; del Álamo, Juan C.; Chien, Shu; Tsai, Jin Wu.

In: Biochemical and Biophysical Research Communications, Vol. 497, No. 3, 11.03.2018, p. 869-875.

Research output: Contribution to journalArticle

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AU - Jheng, Guo Wei

AU - Hur, Sung Sik

AU - Chang, Chia Ming

AU - Wu, Chun Chieh

AU - Cheng, Jia Shing

AU - Lee, Hsiao Hui

AU - Chung, Bon Chu

AU - Wang, Yang-Gao

AU - Lin, Keng Hui

AU - del Álamo, Juan C.

AU - Chien, Shu

AU - Tsai, Jin Wu

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Y1 - 2018/3/11

N2 - Cell migration is a critical process during development, tissue repair, and cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal dynamics, and force generation. Lis1 plays an important role in the migration of neurons, fibroblasts and other cell types, and is essential for normal development of the cerebral cortex. Mutations in human LIS1 gene cause classical lissencephaly (smooth brain), resulting from defects in neuronal migration. However, how Lis1 may affect force generation in migrating cells is still not fully understood. Using traction force microscopy (TFM) with live cell imaging to measure cellular traction force in migrating NIH3T3 cells, we showed that Lis1 knockdown (KD) by RNA interference (RNAi) caused reductions in cell migration and traction force against the extracellular matrix (ECM). Immunostaining of cytoskeletal components in Lis1 KD cells showed disorganization of microtubules and actin filaments. Interestingly, focal adhesions at the cell periphery were significantly reduced. These results suggest that Lis1 is important for cellular traction force generation through the regulation of cytoskeleton organization and focal adhesion formation in migrating cells.

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