Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration

Guo Wei Jheng, Sung Sik Hur, Chia Ming Chang, Chun Chieh Wu, Jia Shing Cheng, Hsiao Hui Lee, Bon Chu Chung, Yang Kao Wang, Keng Hui Lin, Juan C. del Álamo, Shu Chien, Jin Wu Tsai

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Cell migration is a critical process during development, tissue repair, and cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal dynamics, and force generation. Lis1 plays an important role in the migration of neurons, fibroblasts and other cell types, and is essential for normal development of the cerebral cortex. Mutations in human LIS1 gene cause classical lissencephaly (smooth brain), resulting from defects in neuronal migration. However, how Lis1 may affect force generation in migrating cells is still not fully understood. Using traction force microscopy (TFM) with live cell imaging to measure cellular traction force in migrating NIH3T3 cells, we showed that Lis1 knockdown (KD) by RNA interference (RNAi) caused reductions in cell migration and traction force against the extracellular matrix (ECM). Immunostaining of cytoskeletal components in Lis1 KD cells showed disorganization of microtubules and actin filaments. Interestingly, focal adhesions at the cell periphery were significantly reduced. These results suggest that Lis1 is important for cellular traction force generation through the regulation of cytoskeleton organization and focal adhesion formation in migrating cells.

Original languageEnglish
Pages (from-to)869-875
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume497
Issue number3
DOIs
Publication statusPublished - 2018 Mar 11

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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