Localization and expression of the Bacillus subtilis DL-endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes

Yuuka Kiriyama, Kazuya Yazawa, Tatsuhito Tanaka, Ritsuko Yoshikawa, Hisaya Yamane, Masayuki Hashimoto, Junichi Sekiguchi, Hiroki Yamamoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacillus subtilis LytF plays a principal role in cell separation through its localization at the septa and poles on the vegetative cell surface. In this study, we found that a mutation in a major lipoteichoic acid (LTA) synthase gene –ltaS – results in a considerable reduction in the σD-dependent transcription oflytF. The lytF transcription was also reduced in mutants that affected glycolipid anchor biosynthesis. Immunofluorescence microscopy revealed that both the numbers of cells expressing LytF and the LytF foci in these mutants were decreased. In addition, the transcriptional activity of lytF was almost abolished in the double (ltaS yfnI), triple (ltaS yfnI yqgS), and quadruple (ltaS yfnI yqgS yvgJ) mutants during vegetative growth. Cell separation defects in these mutants were partially restored with artificial expression of LytF. Interestingly, when lytF transcription was induced in the ltaS single or multiple mutants, LytF was localized not only at the septum, but also along the sidewall. The amounts of LytF bound to cell wall in the single (ltaS) and double (ltaS yfnI) mutants gradually increased as compared with that in the WT strain, and those in the triple (ltaS yfnI yqgS) and quadruple mutants were almost similar to that in the double mutant. Moreover, reduction of the lytFtranscription and chained cell morphology in the ltaS mutant were completely restored with artificial induction of the yqgS gene. These results strongly suggest that LTA influences the temporal, σD-dependent transcription of lytFand is an additional inhibitory component to the vegetative cell separation enzyme LytF.

Original languageEnglish
Pages (from-to)2639-2649
Number of pages11
JournalMicrobiology (United Kingdom)
Volume160
DOIs
Publication statusPublished - 2014 Jan 1

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Endopeptidases
Cell Separation
Glycolipids
Bacillus subtilis
Mutation
Enzymes
Fluorescence Microscopy
Cell Wall
Genes
Cell Count
Growth
lipoteichoic acid

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Kiriyama, Yuuka ; Yazawa, Kazuya ; Tanaka, Tatsuhito ; Yoshikawa, Ritsuko ; Yamane, Hisaya ; Hashimoto, Masayuki ; Sekiguchi, Junichi ; Yamamoto, Hiroki. / Localization and expression of the Bacillus subtilis DL-endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes. In: Microbiology (United Kingdom). 2014 ; Vol. 160. pp. 2639-2649.
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abstract = "Bacillus subtilis LytF plays a principal role in cell separation through its localization at the septa and poles on the vegetative cell surface. In this study, we found that a mutation in a major lipoteichoic acid (LTA) synthase gene –ltaS – results in a considerable reduction in the σD-dependent transcription oflytF. The lytF transcription was also reduced in mutants that affected glycolipid anchor biosynthesis. Immunofluorescence microscopy revealed that both the numbers of cells expressing LytF and the LytF foci in these mutants were decreased. In addition, the transcriptional activity of lytF was almost abolished in the double (ltaS yfnI), triple (ltaS yfnI yqgS), and quadruple (ltaS yfnI yqgS yvgJ) mutants during vegetative growth. Cell separation defects in these mutants were partially restored with artificial expression of LytF. Interestingly, when lytF transcription was induced in the ltaS single or multiple mutants, LytF was localized not only at the septum, but also along the sidewall. The amounts of LytF bound to cell wall in the single (ltaS) and double (ltaS yfnI) mutants gradually increased as compared with that in the WT strain, and those in the triple (ltaS yfnI yqgS) and quadruple mutants were almost similar to that in the double mutant. Moreover, reduction of the lytFtranscription and chained cell morphology in the ltaS mutant were completely restored with artificial induction of the yqgS gene. These results strongly suggest that LTA influences the temporal, σD-dependent transcription of lytFand is an additional inhibitory component to the vegetative cell separation enzyme LytF.",
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Localization and expression of the Bacillus subtilis DL-endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes. / Kiriyama, Yuuka; Yazawa, Kazuya; Tanaka, Tatsuhito; Yoshikawa, Ritsuko; Yamane, Hisaya; Hashimoto, Masayuki; Sekiguchi, Junichi; Yamamoto, Hiroki.

In: Microbiology (United Kingdom), Vol. 160, 01.01.2014, p. 2639-2649.

Research output: Contribution to journalArticle

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AU - Yazawa, Kazuya

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AU - Yoshikawa, Ritsuko

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AU - Hashimoto, Masayuki

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