LvRas and LvRap are both important for WSSV replication in Litopenaeus vannamei

Yi Ting Tseng, Ramya Kumar, Han-Ching Wang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The white Spot Syndrome Virus (WSSV) is a pathogen that causes huge economic losses in the shrimp-farming industry globally. At the WSSV genome replication stage (12 hpi) in WSSV-infected shrimp hemocytes, activation of the PI3K-Akt-mTOR pathway triggers metabolic changes that resemble the Warburg effect. In shrimp, the upstream regulators of this pathway are still unknown, and in the present study, we isolate, characterize and investigate two candidate factors, i.e. the shrimp Ras GTPase isoforms LvRas and LvRap, both of which are upregulated after WSSV infection. dsRNA silencing experiments show that virus replication is significantly reduced when expression of either of these genes is suppressed. Pretreatment with the Ras inhibitor Salirasib further suggests that LvRas, which is a homolog to a commonly overexpressed human oncoprotein, may be involved in regulating the WSSV-induced Warburg effect. We also show that while both the PI3K-Akt-mTOR and Raf-MEK-ERK pathways are activated by WSSV infection, LvRas appears to be involved only in the regulation of the mTOR pathway.

Original languageEnglish
Pages (from-to)150-160
Number of pages11
JournalFish and Shellfish Immunology
Volume88
DOIs
Publication statusPublished - 2019 May 1

Fingerprint

White spot syndrome virus
Litopenaeus vannamei
virus replication
Viruses
virus
shrimp
phosphatidylinositol 3-kinase
Phosphatidylinositol 3-Kinases
Genes
double-stranded RNA
ras Proteins
guanosinetriphosphatase
hemocytes
Oncogene Proteins
Mitogen-Activated Protein Kinase Kinases
infection
Pathogens
biochemical pathways
farming systems
pretreatment

All Science Journal Classification (ASJC) codes

  • Environmental Chemistry
  • Aquatic Science

Cite this

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title = "LvRas and LvRap are both important for WSSV replication in Litopenaeus vannamei",
abstract = "The white Spot Syndrome Virus (WSSV) is a pathogen that causes huge economic losses in the shrimp-farming industry globally. At the WSSV genome replication stage (12 hpi) in WSSV-infected shrimp hemocytes, activation of the PI3K-Akt-mTOR pathway triggers metabolic changes that resemble the Warburg effect. In shrimp, the upstream regulators of this pathway are still unknown, and in the present study, we isolate, characterize and investigate two candidate factors, i.e. the shrimp Ras GTPase isoforms LvRas and LvRap, both of which are upregulated after WSSV infection. dsRNA silencing experiments show that virus replication is significantly reduced when expression of either of these genes is suppressed. Pretreatment with the Ras inhibitor Salirasib further suggests that LvRas, which is a homolog to a commonly overexpressed human oncoprotein, may be involved in regulating the WSSV-induced Warburg effect. We also show that while both the PI3K-Akt-mTOR and Raf-MEK-ERK pathways are activated by WSSV infection, LvRas appears to be involved only in the regulation of the mTOR pathway.",
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LvRas and LvRap are both important for WSSV replication in Litopenaeus vannamei. / Tseng, Yi Ting; Kumar, Ramya; Wang, Han-Ching.

In: Fish and Shellfish Immunology, Vol. 88, 01.05.2019, p. 150-160.

Research output: Contribution to journalArticle

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