Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro

Ling Yuh Shyu, Trai-Ming Yeh, Han Hsin Chang, David Pei Cheng Lin, Ying Hock Teng, Lien Cheng Chen, Hsiu Hsiung Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalThrombosis Research
Volume129
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

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Macrophage Migration-Inhibitory Factors
Thrombomodulin
Intercellular Adhesion Molecule-1
Human Migration
Blood Coagulation Factors
Endothelial Cells
In Vitro Techniques
Cytokines
Cell Line
Antibodies

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Shyu, Ling Yuh ; Yeh, Trai-Ming ; Chang, Han Hsin ; Lin, David Pei Cheng ; Teng, Ying Hock ; Chen, Lien Cheng ; Lee, Hsiu Hsiung. / Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro. In: Thrombosis Research. 2012 ; Vol. 129, No. 1. pp. 43-49.
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Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro. / Shyu, Ling Yuh; Yeh, Trai-Ming; Chang, Han Hsin; Lin, David Pei Cheng; Teng, Ying Hock; Chen, Lien Cheng; Lee, Hsiu Hsiung.

In: Thrombosis Research, Vol. 129, No. 1, 01.01.2012, p. 43-49.

Research output: Contribution to journalArticle

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AB - Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.

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