Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro

Ling Yuh Shyu; Trai-Ming Yeh; Han Hsin Chang; David Pei Cheng Lin; Ying Hock Teng; Lien Cheng Chen; Hsiu Hsiung Lee

doi:10.1016/j.thromres.2011.08.011

Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro

Ling Yuh Shyu, Trai-Ming Yeh, Han Hsin Chang, David Pei Cheng Lin, Ying Hock Teng, Lien Cheng Chen, Hsiu Hsiung Lee

Department of Medical Laboratory Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Thrombosis Research
Volume	129
Issue number	1
DOIs	https://doi.org/10.1016/j.thromres.2011.08.011
Publication status	Published - 2012 Jan 1

All Science Journal Classification (ASJC) codes

Hematology

Access to Document

10.1016/j.thromres.2011.08.011

Cite this

@article{0c0c37f27f1249558ee40ce2b419fd22,

title = "Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro",

abstract = "Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.",

author = "Shyu, {Ling Yuh} and Trai-Ming Yeh and Chang, {Han Hsin} and Lin, {David Pei Cheng} and Teng, {Ying Hock} and Chen, {Lien Cheng} and Lee, {Hsiu Hsiung}",

year = "2012",

month = jan,

day = "1",

doi = "10.1016/j.thromres.2011.08.011",

language = "English",

volume = "129",

pages = "43--49",

journal = "Thrombosis Research",

issn = "0049-3848",

publisher = "Elsevier Limited",

number = "1",

}

TY - JOUR

T1 - Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro

AU - Shyu, Ling Yuh

AU - Yeh, Trai-Ming

AU - Chang, Han Hsin

AU - Lin, David Pei Cheng

AU - Teng, Ying Hock

AU - Chen, Lien Cheng

AU - Lee, Hsiu Hsiung

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.

AB - Macrophage migration inhibitory factor (MIF) is an important cytokine in the modulation of inflammatory and immune responses, but its role in coagulation remains to be elucidated. In this study, we investigated the potential role of MIF in coagulation through its influence on two factors, thrombomodulin (TM) and intercellular adhesion molecule-1 (ICAM-1). Recombinant human MIF was added to human microvascular endothelial cell line (HMEC-1) to investigate its influence on the expression of TM and ICAM-1. The results showed that both TM and ICAM-1 were induced with MIF addition in a dose-dependent and time-dependent manner. The expression of ICAM-1 and TM was increased as MIF doses were increased, with the highest expression seen at 12 hr after 400 ng/ml of MIF treatment. Besides, anti-MIF antibody treatment reduced the TM expression in HMEC-1 cells. In conclusion, our data support a role of MIF as an important factor in the regulation of coagulation.

UR - http://www.scopus.com/inward/record.url?scp=84655167232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84655167232&partnerID=8YFLogxK

U2 - 10.1016/j.thromres.2011.08.011

DO - 10.1016/j.thromres.2011.08.011

M3 - Article

C2 - 21890178

AN - SCOPUS:84655167232

SN - 0049-3848

VL - 129

SP - 43

EP - 49

JO - Thrombosis Research

JF - Thrombosis Research

IS - 1

ER -

Macrophage migration inhibitory factor induces ICAM-1and thrombomobulin expression in vitro

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this