Malate dehydrogenase is negatively regulated by miR-1 in Trichomonas vaginalis

Wei Chen Lin, Kuo Yang Huang, Shih Chieh Chen, Ting Yun Huang, Shu Jen Chen, Po Jung Huang, Petrus Tang

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

MicroRNAs are highly conserved small noncoding RNAs that can suppress protein translation through complementary binding to target mRNAs. We used a novel approach to identify miRNA targets in the protist Trichomonas vaginalis by comparing the levels of differentially expressed proteins and genes in the trophozoite and amoeboid stages. We observed that the T. vaginalis malate dehydrogenase (Tv-MDH) gene was upregulated 20-fold in the amoeboid stage, but the protein level was reduced by 4.5-fold. Bioinformatics analysis revealed that the Tv-MDH mRNA contains putative target sites of the miR-1 family. The expression level of endogenous tva-miR-1 in the amoeboid stage was 50-fold higher than in the trophozoite stage. Transfection of trophozoites with tva-miR-1 mimics reduced Tv-MDH protein expression by 60%. Based on these experimental data, we conclude that Tv-MDH is negatively regulated by tva-miR-1. The results of this study demonstrate that a combination of proteomic and transcriptomic approaches is a powerful tool for identifying miRNA targets.

Original languageEnglish
Pages (from-to)1683-1689
Number of pages7
JournalParasitology Research
Volume105
Issue number6
DOIs
Publication statusPublished - 2009 Nov

All Science Journal Classification (ASJC) codes

  • Parasitology
  • General Veterinary
  • Insect Science
  • Infectious Diseases

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