MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix

Dabin Lee, Yeoseon Kim, Iqbal Jalaludin, Huu Quang Nguyen, Minsun Kim, Jungju Seo, Kyoung Soon Jang, Jeongkwon Kim

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Disaccharides are sugars composed of two monosaccharides joined by a glycosidic linkage. The specific properties of a disaccharide depend on the type of the glycosidic linkage and the identity of the two component monosaccharides. In this work, seven disaccharide isomers (gentiobiose, isomaltose, melibiose, lactose, maltose, cellobiose, and sucrose) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. Each disaccharide was identified by its unique cleavage pattern. To determine the feasibility of quantitative analyses based on specific fragment patterns, mixtures of sucrose with cellobiose or maltose were prepared at different ratios and analyzed by MALDI-MS, where a strong linear correlation was observed between the relative peak intensity of the sucrose fragment peak at m/z 185 and the amount of sucrose in the mixture. The calibration curve was successfully applied to obtain the relative amount of maltose and sucrose in four different honey samples.

Original languageEnglish
Article number128356
JournalFood Chemistry
Publication statusPublished - 2021 Apr 16

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science


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