TY - JOUR
T1 - MALDI-MS derived prognostic protein markers for resected non-small cell lung cancer
AU - Xu, Baogang J.
AU - Gonzalez, Adriana L.
AU - Kikuchi, Takefumi
AU - Yanagisawa, Kiyoshi
AU - Massion, Pierre P.
AU - Wu, Huiyun
AU - Mason, Stephen E.
AU - Olson, Sandy J.
AU - Shyr, Yu
AU - Carbone, David P.
AU - Caprioli, Richard M.
PY - 2008
Y1 - 2008
N2 - Protein signals obtained directly from frozen lung tissue sections using MALDI-MS were used to predict nodal involvement and survival in resected non-small cell lung cancer (NSCLC). We have identified a list of these protein signals and further evaluated their prognostic values for NSCLC using immunohistochemistry (IHC). Kaplan-Meier analysis was used to assess the mortality risk associated with the prognostic protein IHC-staining intensities. The combined IHC scores of calmodulin, thymosin β4, and thymosin β10 were found to be correlated with NSCLC patient survival (p = 0.004). Furthermore, low cofilin-1 IHC-staining intensity was found to be correlated with a better outcome for patients with negative lymph node status (p = 0.006) while high cofilin-1 IHC-staining intensity was found to be correlated with a better outcome for patients with positive node status (p = 0.034). In conclusion, the prognostic protein signals selected using MALDI-MS can be identified and tested by IHC in formalin-fixed tissue samples. MALDI-MS-derived protein signals can be potentially translated to a conventional clinical setting to aid in the prognosis of patients with NSCLC at the molecular level.
AB - Protein signals obtained directly from frozen lung tissue sections using MALDI-MS were used to predict nodal involvement and survival in resected non-small cell lung cancer (NSCLC). We have identified a list of these protein signals and further evaluated their prognostic values for NSCLC using immunohistochemistry (IHC). Kaplan-Meier analysis was used to assess the mortality risk associated with the prognostic protein IHC-staining intensities. The combined IHC scores of calmodulin, thymosin β4, and thymosin β10 were found to be correlated with NSCLC patient survival (p = 0.004). Furthermore, low cofilin-1 IHC-staining intensity was found to be correlated with a better outcome for patients with negative lymph node status (p = 0.006) while high cofilin-1 IHC-staining intensity was found to be correlated with a better outcome for patients with positive node status (p = 0.034). In conclusion, the prognostic protein signals selected using MALDI-MS can be identified and tested by IHC in formalin-fixed tissue samples. MALDI-MS-derived protein signals can be potentially translated to a conventional clinical setting to aid in the prognosis of patients with NSCLC at the molecular level.
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U2 - 10.1002/prca.200800094
DO - 10.1002/prca.200800094
M3 - Article
C2 - 21136798
AN - SCOPUS:65349121255
SN - 1862-8346
VL - 2
SP - 1508
EP - 1517
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
IS - 10-11
ER -