Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool

Ching Hsiang Fan; Yao Shen Huang; Wei En Huang; Albert Alexander Lee; Sheng Yang Ho; Yu Lin Kao; Cuei Ling Wang; Yen Ling Lian; Tasuku Ueno; Tsung Shing Andrew Wang; Chih Kuang Yeh; Yu Chun Lin

doi:10.1021/acssynbio.7b00162

Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool

Ching Hsiang Fan
, Yao Shen Huang
, Wei En Huang
, Albert Alexander Lee
, Sheng Yang Ho
, Yu Lin Kao
, Cuei Ling Wang
, Yen Ling Lian
, Tasuku Ueno
, Tsung Shing Andrew Wang
, Chih Kuang Yeh
, Yu Chun Lin

Department of Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.

Original language	English
Pages (from-to)	2021-2027
Number of pages	7
Journal	ACS Synthetic Biology
Volume	6
Issue number	11
DOIs	https://doi.org/10.1021/acssynbio.7b00162
Publication status	Published - 2017 Nov 17

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)

Access to Document

10.1021/acssynbio.7b00162

Cite this

@article{c3a7f33724ad4c7a8a75bc2204156116,

title = "Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool",

abstract = "We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.",

author = "Fan, \{Ching Hsiang\} and Huang, \{Yao Shen\} and Huang, \{Wei En\} and Lee, \{Albert Alexander\} and Ho, \{Sheng Yang\} and Kao, \{Yu Lin\} and Wang, \{Cuei Ling\} and Lian, \{Yen Ling\} and Tasuku Ueno and \{Andrew Wang\}, \{Tsung Shing\} and Yeh, \{Chih Kuang\} and Lin, \{Yu Chun\}",

note = "Funding Information: We thank Dr. Takanari Inoue at Johns Hopkins University School of Medicine for sharing the DNA constructs. We are grateful to Dr. Hwan-You Chang at National Tsing Hua University for sharing the C2BBe1 cell lines. This work was partially supported by the Ministry of Science and Technology (MOST), Taiwan (MOST 105-2113-M-002-003 to T.S.A.W., MOST 105-2221-E-007-055 and MOST 105-2119-M-182-001 to C.K.Y., MOST 104-2311-B-007-001, MOST 105-2628-B-007-001-MY3, and Program for Translational Innovation of Biopharmaceutical Development-Technology Supporting Platform Axis-nMACS imaging to Y.C.L., and start-up funds from the National Tsing Hua University to Y.C.L. Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

year = "2017",

month = nov,

day = "17",

doi = "10.1021/acssynbio.7b00162",

language = "English",

volume = "6",

pages = "2021--2027",

journal = "ACS Synthetic Biology",

issn = "2161-5063",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool

AU - Fan, Ching Hsiang

AU - Huang, Yao Shen

AU - Huang, Wei En

AU - Lee, Albert Alexander

AU - Ho, Sheng Yang

AU - Kao, Yu Lin

AU - Wang, Cuei Ling

AU - Lian, Yen Ling

AU - Ueno, Tasuku

AU - Andrew Wang, Tsung Shing

AU - Yeh, Chih Kuang

AU - Lin, Yu Chun

N1 - Funding Information: We thank Dr. Takanari Inoue at Johns Hopkins University School of Medicine for sharing the DNA constructs. We are grateful to Dr. Hwan-You Chang at National Tsing Hua University for sharing the C2BBe1 cell lines. This work was partially supported by the Ministry of Science and Technology (MOST), Taiwan (MOST 105-2113-M-002-003 to T.S.A.W., MOST 105-2221-E-007-055 and MOST 105-2119-M-182-001 to C.K.Y., MOST 104-2311-B-007-001, MOST 105-2628-B-007-001-MY3, and Program for Translational Innovation of Biopharmaceutical Development-Technology Supporting Platform Axis-nMACS imaging to Y.C.L., and start-up funds from the National Tsing Hua University to Y.C.L. Publisher Copyright: © 2017 American Chemical Society.

PY - 2017/11/17

Y1 - 2017/11/17

N2 - We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.

AB - We developed an ultrasound-chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.

UR - https://www.scopus.com/pages/publications/85034608326

UR - https://www.scopus.com/pages/publications/85034608326#tab=citedBy

U2 - 10.1021/acssynbio.7b00162

DO - 10.1021/acssynbio.7b00162

M3 - Article

C2 - 28945972

AN - SCOPUS:85034608326

SN - 2161-5063

VL - 6

SP - 2021

EP - 2027

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

IS - 11

ER -

Manipulating Cellular Activities Using an Ultrasound-Chemical Hybrid Tool

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this