The research of individual cells is an important technique in many kinds of biological study. In addition, cell impedance analysis is an effective way of biological measurement. Therefore, integrating the measurement technique into the single-cell trapping structure is the aim of this work. This study presents a cell manipulation and measurement microchip to achieve a precise positioning which uses the Alternating current electrokinetics (ACET) and negative dielectrophoresis (nDEP) force to move the particle and cell on the measurement electrodes. ACET and DEP are the electrical methods to manipulate particle, and can be easily combined with subsequent analyses based on electric fields. The microwell in the prior study is separated into two parts and regarded as the measurement electrodes. Besides, the original structure has been modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the parameters in the structure. By means of simulations and analyses, the optimum structure for the Hela cell is presented. Finally, the structure was applied to trap and measure the HeLa cell.