TY - GEN
T1 - Manipulation and impedance measurement of single HeLa cells based on the dieletrophoresis microwell
AU - Lan, Kung Chieh
AU - Chen, Ming Kun
AU - Liu, Chia Feng
AU - Jang, Ling Sheng
PY - 2011
Y1 - 2011
N2 - The research of individual cells is an important technique in many kinds of biological study. In addition, cell impedance analysis is an effective way of biological measurement. Therefore, integrating the measurement technique into the single-cell trapping structure is the aim of this work. This study presents a cell manipulation and measurement microchip to achieve a precise positioning which uses the Alternating current electrokinetics (ACET) and negative dielectrophoresis (nDEP) force to move the particle and cell on the measurement electrodes. ACET and DEP are the electrical methods to manipulate particle, and can be easily combined with subsequent analyses based on electric fields. The microwell in the prior study is separated into two parts and regarded as the measurement electrodes. Besides, the original structure has been modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the parameters in the structure. By means of simulations and analyses, the optimum structure for the Hela cell is presented. Finally, the structure was applied to trap and measure the HeLa cell.
AB - The research of individual cells is an important technique in many kinds of biological study. In addition, cell impedance analysis is an effective way of biological measurement. Therefore, integrating the measurement technique into the single-cell trapping structure is the aim of this work. This study presents a cell manipulation and measurement microchip to achieve a precise positioning which uses the Alternating current electrokinetics (ACET) and negative dielectrophoresis (nDEP) force to move the particle and cell on the measurement electrodes. ACET and DEP are the electrical methods to manipulate particle, and can be easily combined with subsequent analyses based on electric fields. The microwell in the prior study is separated into two parts and regarded as the measurement electrodes. Besides, the original structure has been modified for precise positioning. Numerical simulations and analyses are conducted to compute and analyze the effects of the parameters in the structure. By means of simulations and analyses, the optimum structure for the Hela cell is presented. Finally, the structure was applied to trap and measure the HeLa cell.
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U2 - 10.1109/NEMS.2011.6017300
DO - 10.1109/NEMS.2011.6017300
M3 - Conference contribution
AN - SCOPUS:80053305687
SN - 9781612847757
T3 - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
SP - 79
EP - 83
BT - NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
T2 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Y2 - 20 February 2011 through 23 February 2011
ER -