Measurement of cerebral blood flow and oxygen saturation using laser Doppler flowmetry anal near infrared spectroscopy in ischemic stroke rats

Li Fu Liu, Chih Kuang Yeh, Chih-Hung Chen, Tak-Wah Wong, Jia-Jin Chen

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Abstract

Near infrared spectroscopy (NIRS) has been recently developed as a noninvasive technique to assess cerebral oxygenation, hemodynamics and neuronal activity in animals and humans. In the present study, we applied laser Doppler flowmetry (LDF) and NIRS to compare the changes in cerebral blood flow (CBF) with changes of NIRS parameters including concentrations of de/oxygenated hemoglobin (Hb and HbO2), total hemoglobin (HbT) and cerebral oxygen saturation (StO2) in rats subject to transient (60-mins) middle cerebral artery occlusion (MCAO). In addition, NIRS parameters were recorded from the initial ischemic events up to the fourth day after permanent ischemia. Ischemic changes, averaged over the 60 minutes of occlusion, were as follows: ΔCBF = -54.58 ± 6.38%, ΔStO2 = -14.49 ± 2.82%, ΔHbT = -11.66 ± 10.23 μM, ΔHbO2 = -28.47 ± 9.10 μM, ΔHb = 16.82 ± 4.63 μM. Our results revealed good correlation between the changes in laser Doppler flow and StO2. Mismatch between cerebral blood flow and volume was also found. The results of time course monitoring in rats with permanent MCAO showed that the changes in [HbO2] and in StO2 were similar to the behavioral recovery. Our study suggested that NIRS might be a practical tool for noninvasive and continuous monitoring of the hemodynamic condition of ischemic stroke during recovery or for therapeutic schemes.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalJournal of Medical and Biological Engineering
Volume28
Issue number2
Publication statusPublished - 2008 Jun 1

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Near infrared spectroscopy
Rats
Blood
Hemoglobin
Oxygen
Lasers
Hemodynamics
Recovery
Oxygenation
Monitoring
Animals

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering

Cite this

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title = "Measurement of cerebral blood flow and oxygen saturation using laser Doppler flowmetry anal near infrared spectroscopy in ischemic stroke rats",
abstract = "Near infrared spectroscopy (NIRS) has been recently developed as a noninvasive technique to assess cerebral oxygenation, hemodynamics and neuronal activity in animals and humans. In the present study, we applied laser Doppler flowmetry (LDF) and NIRS to compare the changes in cerebral blood flow (CBF) with changes of NIRS parameters including concentrations of de/oxygenated hemoglobin (Hb and HbO2), total hemoglobin (HbT) and cerebral oxygen saturation (StO2) in rats subject to transient (60-mins) middle cerebral artery occlusion (MCAO). In addition, NIRS parameters were recorded from the initial ischemic events up to the fourth day after permanent ischemia. Ischemic changes, averaged over the 60 minutes of occlusion, were as follows: ΔCBF = -54.58 ± 6.38{\%}, ΔStO2 = -14.49 ± 2.82{\%}, ΔHbT = -11.66 ± 10.23 μM, ΔHbO2 = -28.47 ± 9.10 μM, ΔHb = 16.82 ± 4.63 μM. Our results revealed good correlation between the changes in laser Doppler flow and StO2. Mismatch between cerebral blood flow and volume was also found. The results of time course monitoring in rats with permanent MCAO showed that the changes in [HbO2] and in StO2 were similar to the behavioral recovery. Our study suggested that NIRS might be a practical tool for noninvasive and continuous monitoring of the hemodynamic condition of ischemic stroke during recovery or for therapeutic schemes.",
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AU - Liu, Li Fu

AU - Yeh, Chih Kuang

AU - Chen, Chih-Hung

AU - Wong, Tak-Wah

AU - Chen, Jia-Jin

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N2 - Near infrared spectroscopy (NIRS) has been recently developed as a noninvasive technique to assess cerebral oxygenation, hemodynamics and neuronal activity in animals and humans. In the present study, we applied laser Doppler flowmetry (LDF) and NIRS to compare the changes in cerebral blood flow (CBF) with changes of NIRS parameters including concentrations of de/oxygenated hemoglobin (Hb and HbO2), total hemoglobin (HbT) and cerebral oxygen saturation (StO2) in rats subject to transient (60-mins) middle cerebral artery occlusion (MCAO). In addition, NIRS parameters were recorded from the initial ischemic events up to the fourth day after permanent ischemia. Ischemic changes, averaged over the 60 minutes of occlusion, were as follows: ΔCBF = -54.58 ± 6.38%, ΔStO2 = -14.49 ± 2.82%, ΔHbT = -11.66 ± 10.23 μM, ΔHbO2 = -28.47 ± 9.10 μM, ΔHb = 16.82 ± 4.63 μM. Our results revealed good correlation between the changes in laser Doppler flow and StO2. Mismatch between cerebral blood flow and volume was also found. The results of time course monitoring in rats with permanent MCAO showed that the changes in [HbO2] and in StO2 were similar to the behavioral recovery. Our study suggested that NIRS might be a practical tool for noninvasive and continuous monitoring of the hemodynamic condition of ischemic stroke during recovery or for therapeutic schemes.

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