Measurement of diffusion and partition coefficients of ferrocyanide in protein-immobilized membranes

Hsien-Chang Chang, Ching Chou Wu, Shinn Jyh Ding, I. Shiun Lin, I-Wen Sun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

True diffusion (Dm) and partition (α) coefficients for the transport of potassium ferrocyanide through diaphorase (Dp)- and bovine serum albumin (BSA)-glutaraldehyde (GA) membranes with different cross-linking degrees of 1-8% GA concentrations immobilized on gold electrodes are investigated by using potential-step method and rotating-disk-electrode method. The thickness of dry and hydrated immobilized membranes is accurately measured by the focus-difference method with a reflection microscope. The thickness of hydrated Dp-GA and BSA-GA membranes are about 1.4 and 2.4 times that of dry membranes, respectively. In addition, the actual area of electrode surface is calculated by the charge amount of chemisorbed oxygen on gold electrode. Owing to the increase of swelling degree and net negative charge of the immobilized membranes, the values of Dm and α for both of Dp-GA and BSA-GA membranes enlarge and decrease with increase of GA concentration, respectively. Furthermore, BSA-GA membranes possess greater Dm and α than those of Dp-GA membranes due to the thinner thickness and the greater swelling degree of BSA-GA membranes.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalAnalytica Chimica Acta
Volume532
Issue number2
DOIs
Publication statusPublished - 2005 Mar 14

Fingerprint

Immobilized Proteins
Glutaral
partition coefficient
Membrane Proteins
membrane
Membranes
protein
Bovine Serum Albumin
serum
electrode
Electrodes
swelling
Gold
Swelling
gold
hexacyanoferrate II
Rotating disks
potassium
bovine
Microscopes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

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title = "Measurement of diffusion and partition coefficients of ferrocyanide in protein-immobilized membranes",
abstract = "True diffusion (Dm) and partition (α) coefficients for the transport of potassium ferrocyanide through diaphorase (Dp)- and bovine serum albumin (BSA)-glutaraldehyde (GA) membranes with different cross-linking degrees of 1-8{\%} GA concentrations immobilized on gold electrodes are investigated by using potential-step method and rotating-disk-electrode method. The thickness of dry and hydrated immobilized membranes is accurately measured by the focus-difference method with a reflection microscope. The thickness of hydrated Dp-GA and BSA-GA membranes are about 1.4 and 2.4 times that of dry membranes, respectively. In addition, the actual area of electrode surface is calculated by the charge amount of chemisorbed oxygen on gold electrode. Owing to the increase of swelling degree and net negative charge of the immobilized membranes, the values of Dm and α for both of Dp-GA and BSA-GA membranes enlarge and decrease with increase of GA concentration, respectively. Furthermore, BSA-GA membranes possess greater Dm and α than those of Dp-GA membranes due to the thinner thickness and the greater swelling degree of BSA-GA membranes.",
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Measurement of diffusion and partition coefficients of ferrocyanide in protein-immobilized membranes. / Chang, Hsien-Chang; Wu, Ching Chou; Ding, Shinn Jyh; Lin, I. Shiun; Sun, I-Wen.

In: Analytica Chimica Acta, Vol. 532, No. 2, 14.03.2005, p. 209-214.

Research output: Contribution to journalArticle

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