Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system

Ko Wei Chang; Jing Lun Li; Ching Hsuan Yang; Shu Chu Shiesh; Gwo Bin Lee

doi:10.1109/NEMS.2014.6908895

Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system

Ko Wei Chang
, Jing Lun Li
, Ching Hsuan Yang
, Shu Chu Shiesh
, Gwo Bin Lee

Department of Medical Laboratory Science and Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Citations (Scopus)

Abstract

Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.

Original language	English
Title of host publication	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	647-650
Number of pages	4
ISBN (Electronic)	9781479947270
DOIs	https://doi.org/10.1109/NEMS.2014.6908895
Publication status	Published - 2014 Sept 23
Event	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 - Waikiki Beach, United States Duration: 2014 Apr 13 → 2014 Apr 16

Publication series

Name	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Other

Other	9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014
Country/Territory	United States
City	Waikiki Beach
Period	14-04-13 → 14-04-16

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Control and Systems Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/NEMS.2014.6908895

Cite this

Chang, K. W., Li, J. L., Yang, C. H., Shiesh, S. C., & Lee, G. B. (2014). Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 (pp. 647-650). Article 6908895 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2014.6908895

Chang, Ko Wei ; Li, Jing Lun ; Yang, Ching Hsuan et al. / Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system. 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 647-650 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

@inproceedings{0207493bd51b4a9a8ab49f0d316f8fee,

title = "Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system",

abstract = "Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4\%-12.5\% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.",

author = "Chang, \{Ko Wei\} and Li, \{Jing Lun\} and Yang, \{Ching Hsuan\} and Shiesh, \{Shu Chu\} and Lee, \{Gwo Bin\}",

note = "Publisher Copyright: {\textcopyright} 2014 IEEE.; 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014 ; Conference date: 13-04-2014 Through 16-04-2014",

year = "2014",

month = sep,

day = "23",

doi = "10.1109/NEMS.2014.6908895",

language = "English",

series = "9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "647--650",

booktitle = "9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014",

address = "United States",

}

Chang, KW, Li, JL, Yang, CH, Shiesh, SC & Lee, GB 2014, Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system. in 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014., 6908895, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Institute of Electrical and Electronics Engineers Inc., pp. 647-650, 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014, Waikiki Beach, United States, 14-04-13. https://doi.org/10.1109/NEMS.2014.6908895

Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system. / Chang, Ko Wei; Li, Jing Lun; Yang, Ching Hsuan et al.
9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc., 2014. p. 647-650 6908895 (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system

AU - Chang, Ko Wei

AU - Li, Jing Lun

AU - Yang, Ching Hsuan

AU - Shiesh, Shu Chu

AU - Lee, Gwo Bin

PY - 2014/9/23

Y1 - 2014/9/23

N2 - Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.

AB - Measurement of the ratio between glycated hemoglobin (HbA1c) and total hemoglobin (Hb) is now recommended for screening and diagnosing diabetes, in addition to monitoring long-term glycemic control for patient management. The costly and time-consuming traditional assay methods, which involve multi-step, bench-top, and antibody-based immunoassays, have presented major challenges to their clinical utility. In this study, an integrated microfluidic system capable of rapidly measuring the level of HbA1c and Hb in an automated fashion was reported. Moreover, this detection assay used nucleic acid aptamers that exhibited high sensitivity and specificity to Hb and HbA1c to further enhance the robustness of the assay. Combining the advantages of microfluidics and aptamers, this system presents a promising tool for clinical diagnosis and management of diabetes. As a demonstration of its clinical utility, we analyzed patient whole blood samples with clinically-relevant concentrations of HbA1c and Hb (4.4%-12.5% and 12.1 g/dL-14.9 g/dL, respectively) on our integrated microfluidic system.

UR - https://www.scopus.com/pages/publications/84908654118

UR - https://www.scopus.com/pages/publications/84908654118#tab=citedBy

U2 - 10.1109/NEMS.2014.6908895

DO - 10.1109/NEMS.2014.6908895

M3 - Conference contribution

AN - SCOPUS:84908654118

T3 - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

SP - 647

EP - 650

BT - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014

Y2 - 13 April 2014 through 16 April 2014

ER -

Chang KW, Li JL, Yang CH, Shiesh SC, Lee GB. Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system. In 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014. Institute of Electrical and Electronics Engineers Inc. 2014. p. 647-650. 6908895. (9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE-NEMS 2014). doi: 10.1109/NEMS.2014.6908895

Measurement of glycated hemoglobin using an aptamer/antibody assay on an integrated microfluidic system

Abstract

Publication series

Other

UN SDGs

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this