Mechanical control of stem cell differentiation using microengineered matrix

Jianping Fu, Yang-Gao Wang, Michael T. Yang, Ted T. Lee, Christopher S. Chen

Research output: Contribution to conferencePaper

Abstract

In this work, we explore the molecular mechanisms by which local mechanical properties (e.g., rigidity) of the extracellular matrix (ECM) cooperates with soluble cues to regulate lineage commitment of human mesenchymal stem cells (hMSCs). We have established different micropost array substrates that can definitively decouple matrix rigidity from other properties including adhesiveness. We applied these substrates to investigate the influences of matrix rigidity on cell adhesion, cytoskeleton assembly/contractility, cell spreading, and proliferation. We further show that matrix rigidity regulates commitment of hMSCs to either adipogenic or osteogenic fate: soft matrix facilitates adipogenic differentiation while stiff matrix proves osteogenic.

Original languageEnglish
Pages1229-1231
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08-10-1208-10-16

Fingerprint

Stem cells
Rigidity
Cell adhesion
Substrates
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Fu, J., Wang, Y-G., Yang, M. T., Lee, T. T., & Chen, C. S. (2008). Mechanical control of stem cell differentiation using microengineered matrix. 1229-1231. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.
Fu, Jianping ; Wang, Yang-Gao ; Yang, Michael T. ; Lee, Ted T. ; Chen, Christopher S. / Mechanical control of stem cell differentiation using microengineered matrix. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.3 p.
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Fu, J, Wang, Y-G, Yang, MT, Lee, TT & Chen, CS 2008, 'Mechanical control of stem cell differentiation using microengineered matrix' Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 08-10-12 - 08-10-16, pp. 1229-1231.

Mechanical control of stem cell differentiation using microengineered matrix. / Fu, Jianping; Wang, Yang-Gao; Yang, Michael T.; Lee, Ted T.; Chen, Christopher S.

2008. 1229-1231 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conferencePaper

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Fu J, Wang Y-G, Yang MT, Lee TT, Chen CS. Mechanical control of stem cell differentiation using microengineered matrix. 2008. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.