Mechanical control of stem cell differentiation using microengineered matrix

Jianping Fu; Yang Kao Wang; Michael T. Yang; Ted T. Lee; Christopher S. Chen

Mechanical control of stem cell differentiation using microengineered matrix

Jianping Fu, Yang Kao Wang, Michael T. Yang, Ted T. Lee, Christopher S. Chen

Department of Cell Biology and Anatomy

Research output: Contribution to conference › Paper › peer-review

Abstract

In this work, we explore the molecular mechanisms by which local mechanical properties (e.g., rigidity) of the extracellular matrix (ECM) cooperates with soluble cues to regulate lineage commitment of human mesenchymal stem cells (hMSCs). We have established different micropost array substrates that can definitively decouple matrix rigidity from other properties including adhesiveness. We applied these substrates to investigate the influences of matrix rigidity on cell adhesion, cytoskeleton assembly/contractility, cell spreading, and proliferation. We further show that matrix rigidity regulates commitment of hMSCs to either adipogenic or osteogenic fate: soft matrix facilitates adipogenic differentiation while stiff matrix proves osteogenic.

Original language	English
Pages	1229-1231
Number of pages	3
Publication status	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08-10-12 → 08-10-16

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Mechanical control of stem cell differentiation using microengineered matrix",

abstract = "In this work, we explore the molecular mechanisms by which local mechanical properties (e.g., rigidity) of the extracellular matrix (ECM) cooperates with soluble cues to regulate lineage commitment of human mesenchymal stem cells (hMSCs). We have established different micropost array substrates that can definitively decouple matrix rigidity from other properties including adhesiveness. We applied these substrates to investigate the influences of matrix rigidity on cell adhesion, cytoskeleton assembly/contractility, cell spreading, and proliferation. We further show that matrix rigidity regulates commitment of hMSCs to either adipogenic or osteogenic fate: soft matrix facilitates adipogenic differentiation while stiff matrix proves osteogenic.",

author = "Jianping Fu and Wang, {Yang Kao} and Yang, {Michael T.} and Lee, {Ted T.} and Chen, {Christopher S.}",

year = "2008",

language = "English",

pages = "1229--1231",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Mechanical control of stem cell differentiation using microengineered matrix

AU - Fu, Jianping

AU - Wang, Yang Kao

AU - Yang, Michael T.

AU - Lee, Ted T.

AU - Chen, Christopher S.

PY - 2008

Y1 - 2008

N2 - In this work, we explore the molecular mechanisms by which local mechanical properties (e.g., rigidity) of the extracellular matrix (ECM) cooperates with soluble cues to regulate lineage commitment of human mesenchymal stem cells (hMSCs). We have established different micropost array substrates that can definitively decouple matrix rigidity from other properties including adhesiveness. We applied these substrates to investigate the influences of matrix rigidity on cell adhesion, cytoskeleton assembly/contractility, cell spreading, and proliferation. We further show that matrix rigidity regulates commitment of hMSCs to either adipogenic or osteogenic fate: soft matrix facilitates adipogenic differentiation while stiff matrix proves osteogenic.

AB - In this work, we explore the molecular mechanisms by which local mechanical properties (e.g., rigidity) of the extracellular matrix (ECM) cooperates with soluble cues to regulate lineage commitment of human mesenchymal stem cells (hMSCs). We have established different micropost array substrates that can definitively decouple matrix rigidity from other properties including adhesiveness. We applied these substrates to investigate the influences of matrix rigidity on cell adhesion, cytoskeleton assembly/contractility, cell spreading, and proliferation. We further show that matrix rigidity regulates commitment of hMSCs to either adipogenic or osteogenic fate: soft matrix facilitates adipogenic differentiation while stiff matrix proves osteogenic.

UR - http://www.scopus.com/inward/record.url?scp=84902437091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902437091&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902437091

SP - 1229

EP - 1231

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Mechanical control of stem cell differentiation using microengineered matrix

Abstract

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All Science Journal Classification (ASJC) codes

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