Mechanical quantification of the epithelial mesenchymal transition

Tsung Hsien Wu, Jen I. Liang, Yu Wei Chiu, Ming Long Yeh, Chia Hsin Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

During EMT (Epithelial mesenchymal transition), cells are under large shape change. The ingress of the cell relies on the debonding between adjacent epithelium and the flexibility change of them. In order to elucidate the mechanical involvement of EMT, it is necessary to investigate the mechanical properties, cell compressive stiffness and extensibility and the adhesion strength between them. The purpose of this study was using special micropipette to quantify the mechanical property transition when the cell type changed. We built a new special micropipette system which could be used to measure both the tension and compression rigidities of cells before and after EMT. We changed the original design of dual micropipettes with strain gauge cell mechanical system into single micropipette with AFM Cantilever tip for force quantification. We had finished the build up of the micropipette system and AFM tip holder with electronic manipulator. The normal mouse mammary gland cells (NMuMGs) had been successfully grabbed by this novel micropipette system and the force displacement curve for axial compression test of NMuMG had been recorded. However, like the most in house design machine, this novel micropipette mechanical system needs further verification in accuracy and calibration.

Original languageEnglish
Title of host publicationNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
Pages458-461
Number of pages4
DOIs
Publication statusPublished - 2011 Oct 4
Event6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 - Kaohsiung, Taiwan
Duration: 2011 Feb 202011 Feb 23

Publication series

NameNEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems

Other

Other6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011
Country/TerritoryTaiwan
CityKaohsiung
Period11-02-2011-02-23

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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