Mechanical regulation of cell function with geometrically modulated elastomeric substrates

Jianping Fu, Yang Kao Wang, Michael T. Yang, Ravi A. Desai, Xiang Yu, Zhijun Liu, Christopher S. Chen

Research output: Contribution to journalArticle

667 Citations (Scopus)

Abstract

We report the establishment of a library of micromolded elastomeric micropost arrays to modulate substrate rigidity independently of effects on adhesive and other material surface properties. We demonstrated that micropost rigidity impacts cell morphology, focal adhesions, cytoskeletal contractility and stem cell differentiation. Furthermore, early changes in cytoskeletal contractility predicted later stem cell fate decisions in single cells.

Original languageEnglish
Pages (from-to)733-736
Number of pages4
JournalNature Methods
Volume7
Issue number9
DOIs
Publication statusPublished - 2010 Sep 1

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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