Mechanism of hydrodynamic separation of biological objects in microchannel devices

Yu Cheng Lin, Chun Ping Jen

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


In this study, the separation mechanism employed in hydrodynamic chromatography in microchannel devices is analyzed. The main purpose of this work is to provide a methodology to develop a predictive model for hydrodynamic chromatography for biological macromolecules in microchannels and to assess the importance of various phenomenological coefficients. A theoretical model for the hydrodynamic chromatography of particles in a microchannel is investigated herein. A fully developed concentration profile for non-reactive particles in a microchannel was obtained to elucidate the hydrodynamic chromatography of these particles. The external forces acting on the particles considered in this model include the van der Waals attractive force, double-layer force as well as the gravitational force. The surface forces, such as van der Waals attractive force as well as the double-layer repulsive force, can either enhance or hinder the average velocity of the macromolecular particles. The average velocity of the particles decreases with the molecular radius because the van der Waals attractive force increases the concentration of the particles near the channel surface, which is the low-velocity region. The transport velocity of the particles is dominated by the gravity and the higher density enlarges the effect caused by gravity.

Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalLab on a Chip
Issue number3
Publication statusPublished - 2002 Aug 1

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering


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