TY - JOUR
T1 - Mechanism of Toona sinensis-stimulated adrenal steroidogenesis in primary rat adrenal cells
AU - Chen, Yung Chia
AU - Liang, Yi Lin
AU - Huang, Yuan Li
AU - Huang, Bu Miin
N1 - Funding Information:
This study was supported by the National Science Council Taiwan ( NSC102-2320-B-037-008-MY3 ), NCKUH Research Grant ( NCKUH-10102045 ) and Kaohsiung Medical University ( KMU-Q110024 ). We thank professor Hseng-Kuang Hsu for providing TSL-1 extracts and professor Yi-Chen Chia for providing isolated methyl gallate and ethyl gallate.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Toona sinensis is a well-known nutritious plant food widely distributed in Asia. The effects of aqueous leaf extracts of T. sinensis (TSL-1) on steroidogenesis was examined using primary rat adrenal cells. TSL-1 stimulated corticosterone production in a time- and concentration-dependent manner. Cells were then challenged with forskolin, dibutyryl cyclic AMP (dbcAMP), pregnenolone, progesterone or deoxycorticosterone in the presence or absence of TSL-1 for 6 h to determine the mechanism. TSL-1 could enhance forskolin- and dbcAMP-induced steroidogenesis and promote pregnenolone-, progesterone- and deoxycorticosterone-induced steroidogenesis. Moreover, TSL-1 induced steroidogenic acute regulatory protein (StAR) mRNA and protein expression in rat adrenal cells and increased StAR promoter activity in CHO-K1 cells. Thus, the stimulatory effect of TSL-1 was presumed to be mainly due to methyl and ethyl gallates. Therefore, TSL-1-stimulated corticosterone production requires the participation of cAMP-dependent PKA, steroidogenic enzymes and the expression of StAR, which regulates the rate-limiting step in steroidogenesis.
AB - Toona sinensis is a well-known nutritious plant food widely distributed in Asia. The effects of aqueous leaf extracts of T. sinensis (TSL-1) on steroidogenesis was examined using primary rat adrenal cells. TSL-1 stimulated corticosterone production in a time- and concentration-dependent manner. Cells were then challenged with forskolin, dibutyryl cyclic AMP (dbcAMP), pregnenolone, progesterone or deoxycorticosterone in the presence or absence of TSL-1 for 6 h to determine the mechanism. TSL-1 could enhance forskolin- and dbcAMP-induced steroidogenesis and promote pregnenolone-, progesterone- and deoxycorticosterone-induced steroidogenesis. Moreover, TSL-1 induced steroidogenic acute regulatory protein (StAR) mRNA and protein expression in rat adrenal cells and increased StAR promoter activity in CHO-K1 cells. Thus, the stimulatory effect of TSL-1 was presumed to be mainly due to methyl and ethyl gallates. Therefore, TSL-1-stimulated corticosterone production requires the participation of cAMP-dependent PKA, steroidogenic enzymes and the expression of StAR, which regulates the rate-limiting step in steroidogenesis.
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U2 - 10.1016/j.jff.2015.02.012
DO - 10.1016/j.jff.2015.02.012
M3 - Article
AN - SCOPUS:84926340951
SN - 1756-4646
VL - 14
SP - 318
EP - 323
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -