Mechanism of two-photon fluorescence increment via crosslinked bovine serum albumin

C. Y. Lin, C. H. Lien, C. Y. Chang, S. J. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The two-photon excited fluorescence (TPEF) increments of two dyes via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking technique were investigated. One is Rose Bengal (RB) with a high non-radiative decay rate, while the other is Eosin Y with a low non-radiative decay rate. Experimental results demonstrate that the quantum yield and lifetime of RB are both augmented via crosslinked BSA microstructures. Compared with theoretical analysis, this result indicates that the non-radiative decay rate of RB is decreased; hence, the quenched effect induced by BSA solution is suppressed. However, the fluorescence lifetime of Eosin Y is acutely abated despite the augmented quantum yield for the two-photon crosslinking processing from BSA solution. This result deduces that the radiative decay rate increased. Furthermore, the increased TPEF intensity and lifetime of RB correlated with the concentration of fabricated crosslinked BSA microstructures through pulse selection of the employed femtosecond laser is demonstrated and capable of developing a zone-plate-like BSA microstructure.

Original languageEnglish
Title of host publicationPhysical Chemistry of Interfaces and Nanomaterials XI
DOIs
Publication statusPublished - 2012 Dec 1
EventPhysical Chemistry of Interfaces and Nanomaterials XI - San Diego, CA, United States
Duration: 2012 Aug 122012 Aug 15

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8459
ISSN (Print)0277-786X

Conference

ConferencePhysical Chemistry of Interfaces and Nanomaterials XI
CountryUnited States
CitySan Diego, CA
Period12-08-1212-08-15

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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