Mechanosensitive store-operated calcium entry regulates the formation of cell polarity

Yi Wei Huang, Shu Jing Chang, Hans I-Chen Harn, Hui Ting Huang, Hsi Hui Lin, Meng-Ru Shen, Ming-Jer Tang, Wen-Tai Chiu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Ca2+-mediated formation of cell polarity is essential for directional migration which plays an important role in physiological and pathological processes in organisms. To examine the critical role of store-operated Ca2+ entry, which is the major form of extracellular Ca2+ influx in non-excitable cells, in the formation of cell polarity, we employed human bone osteosarcoma U2OS cells, which exhibit distinct morphological polarity during directional migration. Our analyses showed that Ca2+ was concentrated at the rear end of cells and that extracellular Ca2+ influx was important for cell polarization. Inhibition of store-operated Ca2+ entry using specific inhibitors disrupted the formation of cell polarity in a dose-dependent manner. Moreover, the channelosomal components caveolin-1, TRPC1, and Orai1 were concentrated at the rear end of polarized cells. Knockdown of TRPC1 or a TRPC inhibitor, but not knockdown of Orai1, reduced cell polarization. Furthermore, disruption of lipid rafts or overexpression of caveolin-1 contributed to the downregulation of cell polarity. On the other hand, we also found that cell polarity, store-operated Ca2+ entry activity, and cell stiffness were markedly decreased by low substrate rigidity, which may be caused by the disorganization of actin filaments and microtubules that occurs while regulating the activity of the mechanosensitive TRPC1 channel.

Original languageEnglish
Pages (from-to)2086-2097
Number of pages12
JournalJournal of Cellular Physiology
Volume230
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

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Cell Polarity
Caveolin 1
Cells
Calcium
Polarization
Rigidity
Cell-in-Cell Formation
Actins
Bone
Stiffness
Physiological Phenomena
Lipids
Osteosarcoma
Pathologic Processes
Substrates
Actin Cytoskeleton
Microtubules
Down-Regulation
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Huang, Yi Wei ; Chang, Shu Jing ; I-Chen Harn, Hans ; Huang, Hui Ting ; Lin, Hsi Hui ; Shen, Meng-Ru ; Tang, Ming-Jer ; Chiu, Wen-Tai. / Mechanosensitive store-operated calcium entry regulates the formation of cell polarity. In: Journal of Cellular Physiology. 2015 ; Vol. 230, No. 9. pp. 2086-2097.
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Mechanosensitive store-operated calcium entry regulates the formation of cell polarity. / Huang, Yi Wei; Chang, Shu Jing; I-Chen Harn, Hans; Huang, Hui Ting; Lin, Hsi Hui; Shen, Meng-Ru; Tang, Ming-Jer; Chiu, Wen-Tai.

In: Journal of Cellular Physiology, Vol. 230, No. 9, 01.09.2015, p. 2086-2097.

Research output: Contribution to journalArticle

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