TY - JOUR
T1 - Mercury-induced biochemical and proteomic changes in rice roots
AU - Chen, Yun An
AU - Chi, Wen Chang
AU - Huang, Tsai Lien
AU - Lin, Chung Yi
AU - Quynh Nguyeh, Thi Thuy
AU - Hsiung, Yu Chywan
AU - Chia, Li Chiao
AU - Huang, Hao Jen
N1 - Funding Information:
This work was supported by research grants from National Science Council, ROC .
PY - 2012/6
Y1 - 2012/6
N2 - Mercury (Hg) is a serious environmental pollution threats to the planet. Accumulation of Hg in plants disrupts many cellular-level functions and inhibits growth and development, but the mechanism is not fully understood. We investigated cellular, biochemical and proteomic changes in rice roots under Hg stress. Root growth rate was decreased and Hg, reactive oxygen species (ROS), and malondialdehyde (MDA) content and lipoxygenase activity were increased significantly with increasing Hg concentration in roots. We revealed a time-dependent alteration in total glutathione content and enzymatic activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) during Hg stress. 2-D electrophoresis revealed differential expression of 25 spots with Hg treatment of roots: 14 spots were upregulated and 11 spots downregulated. These differentially expressed proteins were identified by ESI-MS/MS to be involved in cellular functions including redox and hormone homeostasis, chaperone activity, metabolism, and transcription regulation. These results may provide new insights into the molecular basis of the Hg stress response in plants.
AB - Mercury (Hg) is a serious environmental pollution threats to the planet. Accumulation of Hg in plants disrupts many cellular-level functions and inhibits growth and development, but the mechanism is not fully understood. We investigated cellular, biochemical and proteomic changes in rice roots under Hg stress. Root growth rate was decreased and Hg, reactive oxygen species (ROS), and malondialdehyde (MDA) content and lipoxygenase activity were increased significantly with increasing Hg concentration in roots. We revealed a time-dependent alteration in total glutathione content and enzymatic activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) during Hg stress. 2-D electrophoresis revealed differential expression of 25 spots with Hg treatment of roots: 14 spots were upregulated and 11 spots downregulated. These differentially expressed proteins were identified by ESI-MS/MS to be involved in cellular functions including redox and hormone homeostasis, chaperone activity, metabolism, and transcription regulation. These results may provide new insights into the molecular basis of the Hg stress response in plants.
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U2 - 10.1016/j.plaphy.2012.03.008
DO - 10.1016/j.plaphy.2012.03.008
M3 - Article
C2 - 22522577
AN - SCOPUS:84859803902
SN - 0981-9428
VL - 55
SP - 23
EP - 32
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -
