Mercury-induced biochemical and proteomic changes in rice roots

Yun An Chen, Wen Chang Chi, Tsai Lien Huang, Chung Yi Lin, Thi Thuy Quynh Nguyeh, Yu Chywan Hsiung, Li Chiao Chia, Hao Jen Huang

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Mercury (Hg) is a serious environmental pollution threats to the planet. Accumulation of Hg in plants disrupts many cellular-level functions and inhibits growth and development, but the mechanism is not fully understood. We investigated cellular, biochemical and proteomic changes in rice roots under Hg stress. Root growth rate was decreased and Hg, reactive oxygen species (ROS), and malondialdehyde (MDA) content and lipoxygenase activity were increased significantly with increasing Hg concentration in roots. We revealed a time-dependent alteration in total glutathione content and enzymatic activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) during Hg stress. 2-D electrophoresis revealed differential expression of 25 spots with Hg treatment of roots: 14 spots were upregulated and 11 spots downregulated. These differentially expressed proteins were identified by ESI-MS/MS to be involved in cellular functions including redox and hormone homeostasis, chaperone activity, metabolism, and transcription regulation. These results may provide new insights into the molecular basis of the Hg stress response in plants.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalPlant Physiology and Biochemistry
Volume55
DOIs
Publication statusPublished - 2012 Jun 1

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

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