TY - JOUR
T1 - Metabolic and energetic aspects of biohydrogen production of Clostridium tyrobutyricum
T2 - The effects of hydraulic retention time and peptone addition
AU - Whang, Liang Ming
AU - Lin, Che An
AU - Liu, I. Chun
AU - Wu, Chao Wei
AU - Cheng, Hai Hsuan
N1 - Funding Information:
The authors would like to acknowledge the financial support from National Science Council of Taiwan under Grant NSC 98-3114-E-006-013 and 100-3113-E-006-017 . We thank Dr. I.C. Tseng of the National Cheng Kung University for the gift of C. tyrobutyricum isolate used in this study.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/9
Y1 - 2011/9
N2 - This study evaluates the microbial metabolism and energy demand in fermentative biohydrogen production using Clostridium tyrobutyricum FYa102 at different hydraulic retention times (HRT) over a period of 1-18h. The hydrogen yield shows a positive correlation with the butyrate yield, the B/A ratio, and the Y H2/2(Y HAc+Y HBu) ratio, but a negative correlation with the lactate yield. A decrease in HRT, which is accompanied by an increased biomass growth, tends to decrease the B/A ratio, due presumably to a higher energy demand for microbial growth. The production of lactate at a low HRT, however, may involve an unfavorable change in e - equiv distribution to result in a reduced hydrogen production. Finally, the relatively high hydrogen yields observed in the bioreactor with the peptone addition may be ascribed to the utilization of peptone as an additional energy and/or amino-acid source, thus reducing the glucose demand for biomass growth during the hydrogen production process.
AB - This study evaluates the microbial metabolism and energy demand in fermentative biohydrogen production using Clostridium tyrobutyricum FYa102 at different hydraulic retention times (HRT) over a period of 1-18h. The hydrogen yield shows a positive correlation with the butyrate yield, the B/A ratio, and the Y H2/2(Y HAc+Y HBu) ratio, but a negative correlation with the lactate yield. A decrease in HRT, which is accompanied by an increased biomass growth, tends to decrease the B/A ratio, due presumably to a higher energy demand for microbial growth. The production of lactate at a low HRT, however, may involve an unfavorable change in e - equiv distribution to result in a reduced hydrogen production. Finally, the relatively high hydrogen yields observed in the bioreactor with the peptone addition may be ascribed to the utilization of peptone as an additional energy and/or amino-acid source, thus reducing the glucose demand for biomass growth during the hydrogen production process.
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U2 - 10.1016/j.biortech.2011.03.101
DO - 10.1016/j.biortech.2011.03.101
M3 - Article
C2 - 21511461
AN - SCOPUS:80051700821
VL - 102
SP - 8378
EP - 8383
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 18
ER -