TY - JOUR
T1 - Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis
AU - Tseng, Chung Chih
AU - Lin, Yu Ju
AU - Liu, Wangta
AU - Lin, Hao Yeh
AU - Chou, Hsin Yu
AU - Thia, Caroline
AU - Wu, Jou Hsien
AU - Chang, Jo Shu
AU - Wen, Zhi Hong
AU - Chang, Jui Jen
AU - David Wang, Hui Min
N1 - Funding Information:
This work is supported by Ministry of Science and Technology (MOST 108-2221-E−005-044 ); Deep Sea Water Industry Development Project ( 10411201110 ); and China Medical University Hospital ( DMR-104-090 ).
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/1
Y1 - 2020/1
N2 - 3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.
AB - 3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.
UR - http://www.scopus.com/inward/record.url?scp=85076252397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076252397&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2019.110993
DO - 10.1016/j.fct.2019.110993
M3 - Article
C2 - 31765702
AN - SCOPUS:85076252397
SN - 0278-6915
VL - 135
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
M1 - 110993
ER -