Metabolic engineering probiotic yeast produces 3S, 3′S-astaxanthin to inhibit B16F10 metastasis

Chung Chih Tseng, Yu Ju Lin, Wangta Liu, Hao Yeh Lin, Hsin Yu Chou, Caroline Thia, Jou Hsien Wu, Jo Shu Chang, Zhi Hong Wen, Jui Jen Chang, Hui Min David Wang

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

3S, 3′S-Astaxanthin is the most powerful antioxidant to scavenge free radicals in the world. In this study, a 3S, 3′S-astaxanthin biosynthesis pathway was constructed in a probiotic yeast, Kluveromyces marxianus, denoted YEAST, and its bioactive metabolites were extracted for biofunctional assessments. The bio-safety examination was achieved by two animal models as following: First, no significant toxic effects on YEAST groups were found in zebrafish; Second, after feeding YEAST for 4 weeks, the rat-groups showed no visible abnormality, and no significant change of the body weight and blood biochemistry tests. The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. Taken together, the 3S, 3′S-astaxanthin producing probiotic yeast is safe to be used in the bio-synthesis of functional and pharmaceutical compounds, which have broad industrial applications on cosmetic, food and feed additive and healthcare.

Original languageEnglish
Article number110993
JournalFood and Chemical Toxicology
Volume135
DOIs
Publication statusPublished - 2020 Jan

All Science Journal Classification (ASJC) codes

  • Food Science
  • Toxicology

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