TY - JOUR
T1 - Metabolic flux network analysis of fermentative hydrogen production
T2 - Using Clostridium tyrobutyricum as an example
AU - Cheng, Hai Hsuan
AU - Whang, Liang Ming
AU - Lin, Che An
AU - Liu, I. Chun
AU - Wu, Chao Wei
N1 - Funding Information:
The authors would like to acknowledge the financial support from the National Science Council of Taiwan under Grant NSC 98-3114-E-006-013 , NSC 100-3113-E-006-017 , and NSC 101-3113-E-006-016 .
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2013/8
Y1 - 2013/8
N2 - This study applies metabolic flux network analysis (MFA) to evaluate the metabolic flux of fermentative hydrogen production (FHP) with the use of Clostridium tyrobutyricum fed with either glucose or lactate/acetate as substrates. The MFA results suggest that hydraulic retention time (HRT) presents significant impact on hydrogen production from glucose. At HRT between 4 and 18h, increase of HRT increased hydrogen production but decreased lactate production, while at HRT below 4h decrease of HRT increased hydrogen production but decreased lactate production. The flux for lactate, butyrate and acetate seemed to affect H2 production, due presumably to their impacts on the balance of NADH, ferredoxin and ATP. It is suggested that the MFA can be a useful tool to provide valuable information for optimization and design of the fermentative hydrogen production process.
AB - This study applies metabolic flux network analysis (MFA) to evaluate the metabolic flux of fermentative hydrogen production (FHP) with the use of Clostridium tyrobutyricum fed with either glucose or lactate/acetate as substrates. The MFA results suggest that hydraulic retention time (HRT) presents significant impact on hydrogen production from glucose. At HRT between 4 and 18h, increase of HRT increased hydrogen production but decreased lactate production, while at HRT below 4h decrease of HRT increased hydrogen production but decreased lactate production. The flux for lactate, butyrate and acetate seemed to affect H2 production, due presumably to their impacts on the balance of NADH, ferredoxin and ATP. It is suggested that the MFA can be a useful tool to provide valuable information for optimization and design of the fermentative hydrogen production process.
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U2 - 10.1016/j.biortech.2013.03.141
DO - 10.1016/j.biortech.2013.03.141
M3 - Article
C2 - 23659760
AN - SCOPUS:84878921343
SN - 0960-8524
VL - 141
SP - 233
EP - 239
JO - Bioresource technology
JF - Bioresource technology
ER -
