Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

I. Cheng Chou, Yee-Lin Wu, Lin Chi Wang, Guo Ping Chang-Chien, Hsinyu Lee

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium).

Original languageEnglish
Pages (from-to)881-885
Number of pages5
JournalJournal of Hazardous Materials
Volume165
Issue number1-3
DOIs
Publication statusPublished - 2009 Jun 15

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Environmental Chemistry
  • Environmental Engineering

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