Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

I. Cheng Chou; Yee-Lin Wu; Lin Chi Wang; Guo Ping Chang-Chien; Hsinyu Lee

doi:10.1016/j.jhazmat.2008.10.099

Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells

I. Cheng Chou, Yee-Lin Wu, Lin Chi Wang, Guo Ping Chang-Chien, Hsinyu Lee

Department of Environmental Engineering

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As⁵⁺, Cr⁶⁺, Cd²⁺, and Pb²⁺) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr⁶⁺ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr⁶⁺ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr⁶⁺concentrations (2.0-5.0 μg-Cr/mL-medium).

Original language	English
Pages (from-to)	881-885
Number of pages	5
Journal	Journal of Hazardous Materials
Volume	165
Issue number	1-3
DOIs	https://doi.org/10.1016/j.jhazmat.2008.10.099
Publication status	Published - 2009 Jun 15

All Science Journal Classification (ASJC) codes

Health, Toxicology and Mutagenesis
Pollution
Waste Management and Disposal
Environmental Chemistry
Environmental Engineering

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@article{48ade49dc5d246a3b75eb0975d72a259,

title = "Metal interference on luciferase activity induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in bioassays of recombinant mouse hepatoma cells",

abstract = "This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium).",

author = "Chou, {I. Cheng} and Yee-Lin Wu and Wang, {Lin Chi} and Chang-Chien, {Guo Ping} and Hsinyu Lee",

year = "2009",

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doi = "10.1016/j.jhazmat.2008.10.099",

language = "English",

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AU - Chou, I. Cheng

AU - Wu, Yee-Lin

AU - Wang, Lin Chi

AU - Chang-Chien, Guo Ping

AU - Lee, Hsinyu

PY - 2009/6/15

Y1 - 2009/6/15

N2 - This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium).

AB - This study will examine the effect of a series of metals on the luciferase activity expression induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in H1L6.1 cells. A 2-level factorial design was employed to identify major influential metal species of four test metals (As5+, Cr6+, Cd2+, and Pb2+) in the chemically activated luciferase expression (CALUX) of H1L6.1 cells. The observed data revealed that Cr6+ exerted a significant inhibiting effect on the luciferase activity. The impact of key metal species at various concentrations was analyzed for determination of the maximum tolerable level for H1L6.1 cell. It was observed that Cr6+ lowered the luciferase activity by 36.1 and 65.4%, at levels of 1.0 and 1.5 μg-Cr/mL-medium (p < 0.05), respectively, but showed no significant difference at higher Cr6+concentrations (2.0-5.0 μg-Cr/mL-medium).

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