TY - JOUR
T1 - Metallo-β-lactamase-producing Enterobacteriaceae isolates in a university hospital in Taiwan
T2 - Prevalence of IMP-8 in Enterobacter cloacae and first identification of VIM-2 in Citrobacter freundii
AU - Yan, Jing Jou
AU - Ko, Wen Chien
AU - Chuang, Chin Luan
AU - Wu, Jiunn Jong
N1 - Funding Information:
The authors are grateful to Dr G. M. Rossolini for providing the IMP-2-containing A. baumannii strain AC-54/97 and the VIM-1-containing P. aeruginosa strain VR-143/97. This work was partially supported by grant DOH91-DC1033 from the Center for Disease Control, the Department of Health, the Executive Yuan, and grant NSC 91-2314-B-006-002 from the National Science Council, Taiwan.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - A total of 9082 clinical isolates of Enterobacteriaceae other than Klebsiella spp. collected in 1999 and 2000 at a university hospital in Taiwan were investigated for the production of metallo-β-lactamases (MBLs). Thirty-six (2.9%) of the 1261 Enterobacter cloacae isolates and one (0.3%) of the 340 Citrobacter freundii isolates were found to carry blaIMP-8 and blaVIM-2, respectively, by colony hybridization, PCR and sequence analysis. The IMP-8 producers were recovered from 20 patients and four of them had recently transferred from other hospitals, implying spread of IMP-8-producing E. cloacae among different healthcare settings. Of the 20 non-repetitive IMP-8 producers, 17 (85%) isolates also harboured blaSHV-12, which was on the same transferable plasmids with blaIMP-8. The blaVIM-2-positive isolate and all non-repetitive blaIMP-8-positive isolates appeared susceptible to imipenem (MICs < 8 mg/L) and meropenem (MICs < 4 mg/L), indicating the difficulty in detection of MBLs in Enterobacteriaceae by routine susceptibility testing. Ribotyping of the IMP-8-producing E. cloacae isolates indicated that the dissemination of blaIMP-8 was due largely to the spread of an epidemic clone, but horizontal transfer among unrelated strains also occurred.
AB - A total of 9082 clinical isolates of Enterobacteriaceae other than Klebsiella spp. collected in 1999 and 2000 at a university hospital in Taiwan were investigated for the production of metallo-β-lactamases (MBLs). Thirty-six (2.9%) of the 1261 Enterobacter cloacae isolates and one (0.3%) of the 340 Citrobacter freundii isolates were found to carry blaIMP-8 and blaVIM-2, respectively, by colony hybridization, PCR and sequence analysis. The IMP-8 producers were recovered from 20 patients and four of them had recently transferred from other hospitals, implying spread of IMP-8-producing E. cloacae among different healthcare settings. Of the 20 non-repetitive IMP-8 producers, 17 (85%) isolates also harboured blaSHV-12, which was on the same transferable plasmids with blaIMP-8. The blaVIM-2-positive isolate and all non-repetitive blaIMP-8-positive isolates appeared susceptible to imipenem (MICs < 8 mg/L) and meropenem (MICs < 4 mg/L), indicating the difficulty in detection of MBLs in Enterobacteriaceae by routine susceptibility testing. Ribotyping of the IMP-8-producing E. cloacae isolates indicated that the dissemination of blaIMP-8 was due largely to the spread of an epidemic clone, but horizontal transfer among unrelated strains also occurred.
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M3 - Article
C2 - 12356794
AN - SCOPUS:0036798669
SN - 0305-7453
VL - 50
SP - 503
EP - 511
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 4
ER -