Methanogenic degradation of tetramethylammonium hydroxide by Methanomethylovorans and Methanosarcina

Tai Ho Hu, Liang-Ming Whang, Chi Yu Huang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study evaluated the methanogens responsible for methanogenic degradation of tetramethylammonium hydroxide (TMAH) in a continuous flow bioreactor. The enriched methanogens attained an estimated maximum specific TMAH degradation rate and half-saturation constant of 39.5 mg TMAH/gVSS/h and 820 mg/L, following the Monod-type kinetic expression for methanogenic TMAH degradation. Presence of sulfide more than 20 mg/L significantly extended lag period and slowed down specific TMAH degradation rates. The results of terminal restriction fragment length polymorphism (T-RFLP), cloning/sequencing, and quantitative real-time PCR analyses targeting on the methyl coenzyme M reductase alpha subunit (mcrA) genes retrieved from the bioreactor and batch experiments indicated that Methanomethylovorans species were the dominant methanogens responsible for methanogenic degradation of TMAH. The isolated TMAH-degrading methanogen from the bioreactor, however, was identified closely related to Methanosarcina mazei. It is likely that a very low TMAH environment in the bioreactor favored the growth of Methanomethylovorans hollandica, while the much higher TMAH in the isolation growth medium proliferated Methanosarcina mazei.

Original languageEnglish
Pages (from-to)180-186
Number of pages7
JournalJournal of Hazardous Materials
Volume357
DOIs
Publication statusPublished - 2018 Sep 5

Fingerprint

Methanosarcina
Methanogens
hydroxide
Bioreactors
Degradation
degradation
bioreactor
Coenzymes
Cloning
Polymorphism
Genes
tetramethylammonium
Kinetics
Sulfides
Growth
Restriction Fragment Length Polymorphisms
targeting
Organism Cloning
Real-Time Polymerase Chain Reaction
polymorphism

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

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abstract = "This study evaluated the methanogens responsible for methanogenic degradation of tetramethylammonium hydroxide (TMAH) in a continuous flow bioreactor. The enriched methanogens attained an estimated maximum specific TMAH degradation rate and half-saturation constant of 39.5 mg TMAH/gVSS/h and 820 mg/L, following the Monod-type kinetic expression for methanogenic TMAH degradation. Presence of sulfide more than 20 mg/L significantly extended lag period and slowed down specific TMAH degradation rates. The results of terminal restriction fragment length polymorphism (T-RFLP), cloning/sequencing, and quantitative real-time PCR analyses targeting on the methyl coenzyme M reductase alpha subunit (mcrA) genes retrieved from the bioreactor and batch experiments indicated that Methanomethylovorans species were the dominant methanogens responsible for methanogenic degradation of TMAH. The isolated TMAH-degrading methanogen from the bioreactor, however, was identified closely related to Methanosarcina mazei. It is likely that a very low TMAH environment in the bioreactor favored the growth of Methanomethylovorans hollandica, while the much higher TMAH in the isolation growth medium proliferated Methanosarcina mazei.",
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Methanogenic degradation of tetramethylammonium hydroxide by Methanomethylovorans and Methanosarcina. / Hu, Tai Ho; Whang, Liang-Ming; Huang, Chi Yu.

In: Journal of Hazardous Materials, Vol. 357, 05.09.2018, p. 180-186.

Research output: Contribution to journalArticle

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