Methionine restriction alters bone morphology and affects osteoblast differentiation

Amadou Ouattara, Diana Cooke, Raj Gopalakrishnan, Tsang-Hai Huang, Gene P. Ables

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate the effect of MR on bone structure in young and aged male and female C57BL/6J mice. This study indicated that MR affected the growth rates of males and young females, but not aged females. MR reduced volumetric bone mass density (vBMD) and bone mineral content (BMC), while bone microarchitecture parameters were decreased in males and young females, but not in aged females compared to control-fed (CF) mice. However, when adjusted for bodyweight, the effect of MR in reducing vBMD, BMC and microarchitecture measurements was either attenuated or reversed suggesting that the smaller bones in MR mice is appropriate for its body size. In addition, CF and MR mice had similar intrinsic strength properties as measured by nanoindentation. Plasma biomarkers suggested that the low bone mass in MR mice could be due to increased collagen degradation, which may be influenced by leptin, IGF-1, adiponectin and FGF21 hormone levels. Mouse preosteoblast cell line cultured under low sulfur amino acid growth media attenuated gene expression levels of Col1al, Runx2, Bglap, Alpl and Spp1 suggesting delayed collagen formation and bone differentiation. Collectively, our studies revealed that MR altered bone morphology which could be mediated by delays in osteoblast differentiation.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalBone Reports
Volume5
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Osteoblasts
Methionine
Bone and Bones
Bone Density
Collagen
Growth
Sulfur Amino Acids
Adiponectin
Body Size
Leptin
Insulin-Like Growth Factor I
Inbred C57BL Mouse
Osteogenesis
Drosophila
Rodentia
Yeasts
Biomarkers
Hormones
Gene Expression
Cell Line

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Ouattara, Amadou ; Cooke, Diana ; Gopalakrishnan, Raj ; Huang, Tsang-Hai ; Ables, Gene P. / Methionine restriction alters bone morphology and affects osteoblast differentiation. In: Bone Reports. 2016 ; Vol. 5. pp. 33-42.
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Methionine restriction alters bone morphology and affects osteoblast differentiation. / Ouattara, Amadou; Cooke, Diana; Gopalakrishnan, Raj; Huang, Tsang-Hai; Ables, Gene P.

In: Bone Reports, Vol. 5, 01.12.2016, p. 33-42.

Research output: Contribution to journalArticle

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