Abstract
A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.
| Translated title of the contribution | 葉酸檢測方法及其檢測套組 |
|---|---|
| Original language | English |
| Patent number | 8663946 |
| Publication status | Published - 2012 Aug 16 |