Method and kit for detecting folate

Tzu-Fun Fu (Inventor)

Research output: Patent

Abstract

A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.
Original languageEnglish
Patent number8663946
Publication statusPublished - 2012 Aug 16

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Folic Acid
Catalysis
Centrifugation
High performance liquid chromatography
Hydrolases
Buffers
Cooling
Heating
Enzymes

Cite this

Fu, T-F. (2012). Method and kit for detecting folate. (Patent No. 8663946).
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Fu, T-F 2012, Method and kit for detecting folate, Patent No. 8663946.

Method and kit for detecting folate. / Fu, Tzu-Fun (Inventor).

Patent No.: 8663946.

Research output: Patent

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PY - 2012/8/16

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N2 - A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.

AB - A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.

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Fu T-F, inventor. Method and kit for detecting folate. 8663946. 2012 Aug 16.