Method and kit for detecting folate

Tzu-Fun Fu (Inventor)

Research output: Patent


A method and a kit for detecting folate are disclosed. The method includes the following steps: (a) mixing a sample and an extraction buffer to form a mixture, heating and then cooling the mixture, and separating a supernatant from the mixture by centrifugation; (b) adding a recombinant γ-glutamyl hydrolase (GGH) and a folate conversion enzyme to the supernatant to drive catalysis; (c) stopping the catalysis; and (d) analyzing the supernatant by high performance liquid chromatography.
Translated title of the contribution葉酸檢測方法及其檢測套組
Original languageEnglish
Patent number8663946
Publication statusPublished - 2012 Aug 16


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