Method for detecting mitochondria alterations

Dar-Bin Shieh (Inventor)

Research output: Patent


The present invention relates to a method for detecting mitochondria alterations, which comprises the following steps: (A) providing a separation element and a sample; (B) mixing the separation element and the sample, wherein a detecting sample is obtained through the binding of a DNA fragment on the separation element to mitochondrial DNA in the sample; (C) dividing the detecting sample into a comparison group and a detection group; (D) adding an amplification solution into the comparison group and the detection group respectively to begin a DNA amplified reaction, and further adding a restriction enzyme into the detection group, wherein the amplification solution comprises a labeling reagent and a primer pair; and (E) detecting amounts of the labeling reagent in the comparison group and the detection group respectively after the DNA amplified reaction.
Translated title of the contribution粒線體基因變異之檢測方法
Original languageEnglish
Patent number9551665
Publication statusPublished - 2014 Apr 3


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